QUANTUM EFFICIENCIES OF FLUORESCENCE OF ORGANIC SUBSTANCES - EFFECT OF SOLVENT AND CONCENTRATION OF FLUORESCENT SOLUTE
QUANTUM EFFICIENCIES OF FLUORESCENCE OF ORGANIC SUBSTANCES - EFFECT OF SOLVENT AND CONCENTRATION OF FLUORESCENT SOLUTE
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DOI：
10.1021/j100820a009
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发表时间：
1961-01-01
影响因子：
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通讯作者：
MELHUISH, WH
中科院分区：
文献类型：
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作者：
MELHUISH, WH
用罗丹明B量子计数器测量了几种有机荧光物质在石油醚(bp 60-80)、乙醇(99%)和苯中的脱气溶液在25℃下的荧光绝对量子效率。导出了一个方程，用于校正观察到的荧光重吸收效率和吸收区域远离量子计数器的偏移。外推至零浓度的荧光绝对量子效率、双分子自猝灭常数和氧猝灭常数在 25 时测量。硫酸奎宁，5 X 10” 3 M 在 1N H2SO4 中，建议作为标准品，在 25 时绝对量子荧光效率为 0.51。了解荧光的量子效率和自猝灭常数 溶液中的有机分子在物理化学中具有许多重要的应用，例如，在研究溶液中的内转化、系间窜越（单重态-三重态跃迁）、荧光猝灭和电子激发能的转移等方面。福斯特最近对量子荧光效率的测量进行了综述。 3 Bowen4的方法具有简单的优点，如果修改的话，可以使用5 波长范围广。自 1951 年以来，其他测量荧光效率的方法已经发布。
The absolute quantum efficiencies of fluorescence of deaerated solutions of several organic fluorescent substances in pe-troleum ether (bp 60-80), ethanol (99%) and benzene at 25 were measured with a rhodamin B quantum counter. An equation was derived for correcting the observed efficiencies of fluorescence for reabsorption of fluorescence and shifts in the region of absorption away from the quantumcounter. Absolute quantum efficiencies of fluorescence extrapolated to zero concentration, bimolecular self quenching constants and oxygen-quenching constants were measured at 25. Quinine bisulfate, 5 X 10” 3 M in 1N H2SO4, is proposed as a standard with an absolute quantum fluorescence efficiency of 0.51 at 25.A knowledge of quantum efficiencies and self-quenching constants of fluorescent organic mole-cules in solution has many important applications in physical chemistry, for example, in the investigation of internal conversion, intersystem crossing (singlet-triplet transitions), fluorescence quenching and the transfer of electronic excita-tion energy in solution. The measurement of quantum fluorescence efficiencies has been reviewed recently by Forster. 3 The method of Bowen4 has the merit of simplicity and if modified, 5 may be used over a wide range of wave lengths. Since 1951 other methods of measuring fluorescence efficiencies have been pub-