Anemia induced by repeated exposure to cyanobacterial extracts with explorations of underlying mechanisms
Anemia induced by repeated exposure to cyanobacterial extracts with explorations of underlying mechanisms
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DOI：
10.1002/tox.20583
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发表时间：
2011-10
影响因子：
4.5
通讯作者：
Xuezhen Zhang;P. Xie;Dapeng Li;Z. Shi;Jun Wang;Gailing Yuan;Yanyan Zhao;R. Tang
中科院分区：
文献类型：
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作者：
Xuezhen Zhang;P. Xie;Dapeng Li;Z. Shi;Jun Wang;Gailing Yuan;Yanyan Zhao;R. Tang
1996年在巴西卡鲁阿鲁的血液透析中心，患有微囊藻毒素（MC）的患者出现血液学异常或紊乱。但有关微囊藻毒素血液学效应的实验研究尚不多见，其机制尚不清楚。本研究以6 μg/kg/d的剂量连续14 d腹腔注射家兔（Oryctolagus cuniculus），观察微囊藻毒素对家兔血液毒性的影响。暴露14天后，观察到血液学指标红细胞计数、红细胞压积、血红蛋白和血小板计数显著降低，而家兔出现明显贫血。此外，红细胞体积分布宽度、平均红细胞体积和平均红细胞血红蛋白没有显著变化，表明家兔患有正常细胞性贫血。骨髓细胞微核率在染毒后第14天显著高于对照组，细胞活力显著低于对照组。血清促红细胞生成素水平在第7天和第14天下降，表明红细胞调节分化和成熟的能力受损。结果表明，反复接触微囊藻毒素可导致正常细胞性贫血，骨髓损伤和促红细胞生成素水平的急剧下降是造成贫血的原因。© 2011 Wiley Periodicals，Inc.Environ Toxicol，2011。
Hematological abnormalities or derangements have been demonstrated in patients suffering form microcystins (MCs) in hemodialysis unit in Caruaru, Brazil, 1996. While experimental study on hematological effect of microcystins has been rare and the underlying mechanisms are still puzzling. In the present study, microcystins were repeatedly intraperitoneally injected with a dose of 6 μg/kg/day in rabbits (Oryctolagus cuniculus) for 14 days, and the prolonged effects of extracted microcystins on hematotoxicology were investigated. Significant decreases were observed in the hematological indices red blood cell counts, hematocrit, hemoglobin, and platelet count, while an obvious anemia occurred in rabbits after 14‐day exposure. Moreover, red blood cell volume distribution width, mean corpuscular volume, and mean corpuscular hemoglobin did not vary significantly, indicating that rabbits suffered from normocytic anemia. In bone marrow, on the 14th day after toxin exposure, the frequency of micronucleus increased significantly, and the viability of bone marrow cells decreased markedly compared with the control. Serum erythropoietin levels declined on the 7th and 14th day, which suggested that the ability to regulate differentiation and maturation of erythrocytes was impaired. These results indicate that repeated exposure of microcystins can result in normocyte anemia, and the bone marrow injures and the sharp decreases of erythropoietin levels were responsible for the anemia. © 2011 Wiley Periodicals, Inc.Environ Toxicol, 2011.