Depot-specific differences in perilipin mRNA but not protein expression in obesity
Depot-specific differences in perilipin mRNA but not protein expression in obesity
复制标题
DOI：
10.1111/j.1365-2796.2004.01314.x
复制
发表时间：
2004-05-01
影响因子：
11.1
通讯作者：
Rydén, M
中科院分区：
文献类型：
--
作者：
Arvidsson, E;Blomqvist, L;Rydén, M
背景。 Perilipin (PLIN) 是一种脂滴相关蛋白，通过调节激素敏感脂肪酶进入脂质表面来调节啮齿动物脂肪细胞的脂肪分解。关于 PLIN 在人类中的表达和功能知之甚少。皮下（s.c.）和网膜脂肪组织在基础脂肪分解和刺激脂肪分解方面表现出显着差异。目标和受试者。为了评估这是否是由 PLIN 表达差异引起的，我们研究了皮下 PLIN mRNA 和蛋白质的表达。以及来自 16 名肥胖女性 (n=8) 和男性 (n=8) 的网膜脂肪组织样本。方法。对于 mRNA 检测，使用定量实时 PCR。通过蛋白质印迹分析蛋白质。结果。与皮下相比，网膜中的 PLIN mRNA 显着减少。组织（P=0.022）。相比之下，尽管当与参考蛋白β-肌动蛋白相关时，两个储存库之间的PLIN蛋白水平差异很大，但在脂肪储存库之间没有观察到PLIN蛋白表达的显着差异（P = 0.61）。 PLIN mRNA 或蛋白质不存在性别特异性差异。结论。我们的结果表明，转录后机制可能会影响 PLIN 蛋白表达，并且 PLIN 表达本身可能不是决定皮下脂肪分解差异的主要因素。和网膜脂肪库。
Background. Perilipin (PLIN) is a lipid droplet-associated protein, which regulates lipolysis in rodent adipocytes by modulating the access of hormone-sensitive lipase to the lipid surface. Little is known regarding the expression and function of PLIN in man. Subcutaneous (s.c.) and omental fat tissue display marked differences in both basal and stimulated lipolysis.Objective and subjects. In order to assess whether this can be caused by differences in PLIN expression, we investigated the expression of PLIN mRNA and protein in s.c. and omental fat tissue samples from 16 obese women (n=8) and men (n=8).Methods. For mRNA detection a quantitative real-time PCR was used. Protein was analysed by Western blot.Results. PLIN mRNA was significantly reduced in omental versus s.c. tissue (P=0.022). In contrast, although PLIN protein levels were highly variable between the two depots when related to the reference protein beta-actin, no significant difference in PLIN protein expression was observed between the fat depots (P=0.61). There were no gender-specific differences in PLIN mRNA or protein.Conclusion. Our results suggest that post-transcriptional mechanisms may influence PLIN protein expression and that PLIN expression per se is probably not a major factor in determining the lipolytic differences between the s.c. and omental fat depots.