SYNTHESIS OF NERVE GROWTH-FACTOR BY L AND 3T3 CELLS IN CULTURE
SYNTHESIS OF NERVE GROWTH-FACTOR BY L AND 3T3 CELLS IN CULTURE
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DOI：
10.1073/pnas.71.4.1554
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
YOUNG, M
中科院分区：
文献类型：
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作者：
OGER, J;ARNASON, BGW;YOUNG, M
小鼠颌下腺神经生长因子（NGF）已被共价连接到噬菌体和所得的噬菌体缀合物保持生物活性，刺激神经突起从感觉神经节的延伸。已经开发了灵敏的噬菌体免疫测定法来测量低至1 ng/ml的NGF浓度。用这种方法，我们发现，小鼠L和3 T3细胞培养产生一种生物活性的神经生长因子，这是免疫相似的，如果不相同的小鼠颌下腺神经生长因子。由于已知L细胞是用于组织培养的“条件培养基”的来源，因此这些细胞分泌的一种或多种条件因子活性可能是由于NGF本身。
Mouse submaxillary gland nerve growth factor (NGF) has been covalently joined to bacteriophage and the resulting phage conjugates remain biologically active in stimulating neurite extension from sensory ganglia. A sensitive bacteriophage immunoassay has been developed to measure concentrations of NGF as low as 1 ng/ml. With this method, we find that mouse L and 3T3 cells in culture produce a biologically active nerve growth factor that is immunologically similar if not identical to mouse submaxillary gland NGF. Since L cells are known to be a source of “conditioned medium” for tissue culture, it could be that one or more of the conditioning factor activities secreted by these cells are due to NGF itself.