In vitro membrane damage induced by half-fin anchovy hydrolysates/glucose Maillard reaction products and the effects on oxidative status in vivo.
In vitro membrane damage induced by half-fin anchovy hydrolysates/glucose Maillard reaction products and the effects on oxidative status in vivo.
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DOI：
10.1039/c7fo01459g
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发表时间：
2018-02
期刊：
影响因子：
6.1
通讯作者：
Ru Song;Qingqing Shi;Peiyu Yang;Rong-bian Wei
中科院分区：
文献类型：
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作者：
Ru Song;Qingqing Shi;Peiyu Yang;Rong-bian Wei
使用体外大肠杆菌 (E. coli) 模型评估半鳍鳀鱼水解物和葡萄糖的美拉德反应产物（指定为 HAHp(9.0)-G MRPs）造成的膜损伤。 HAHp(9.0)-G MRP 的孵育导致外膜和内膜通透性显着增加。此外，通过扫描电子显微镜观察到，4',6-二脒基-2-苯基吲哚染色剂的荧光信号减少表明，膜完整性被严重破坏。有趣的是，HAHp(9.0)-G MRPs在孵育3小时后诱导大肠杆菌产生大量过氧化氢(H2O2)，这有助于HAHp(9.0)-G MRPs的抗菌活性。作为活性氧之一，体内过量的 H2O2 可能会损害抗氧化平衡。因此，对健康小鼠短期摄入HAHp(9.0)-G MRPs后的氧化状态进行了研究。低剂量（0.1 g/kg/天体重）和高剂量（1.0 g/kg/天体重）HAHp(9.0)-G MRPs给药14天后，雌性小鼠体重下降，雄性小鼠体重增加。然而，HAHp(9.0)-G MRPs 的施用并不影响雌性或雄性小鼠的肝脏抗氧化防御。检测到一些积极反应，例如超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化物酶 (GPx) 增加以及脂质过氧化 (LPO) 含量降低，特别是在肝脏中。虽然过氧化氢酶活性的降低表明HAHp(9.0)-G MRPs的糖化化合物可能在体内被吸收，但HAHp(9.0)-G MRPs喂养组的SOD/GPx比值低于正常组也表明给予HAHp(9.0)-G MRPs可以减轻小鼠的LPO应激。我们的结果表明，健康动物在喂食 HAHp(9.0)-G MRPs 14 天后可以产生更高的抗氧化状态。
Membrane damage via the Maillard reaction products of half-fin anchovy hydrolysates and glucose (designated as HAHp(9.0)-G MRPs) was evaluated using an in vitro Escherichia coli (E. coli) model. The incubation of HAHp(9.0)-G MRPs led to a significant increase in outer and inner membrane permeability. Furthermore, membrane integrity was seriously destroyed, as observed by scanning electron microscopy and indicated by the decreased fluorescence signal of 4',6-diamidino-2-phenylindole stain. Interestingly, HAHp(9.0)-G MRPs induced significant hydrogen peroxide (H2O2) production in E. coli after 3 h of incubation, which contributed to the antibacterial activity of HAHp(9.0)-G MRPs. As one of the reactive oxygen species, excess H2O2in vivo might impair the antioxidant balance. Therefore, the oxidative status of healthy mice after short-term intake of HAHp(9.0)-G MRPs was investigated. After the administration of HAHp(9.0)-G MRPs at low dose (0.1 g per kg per day body weight) and high dose (1.0 g per kg per day body weight) for 14 days, the body weight of female mice decreased, and the body weight of male mice increased. However, the administration of HAHp(9.0)-G MRPs did not affect the hepatic antioxidant defense in either female or male mice. Some positive responses, like increased superoxide dismutase (SOD) and glutathione peroxidase (GPx) as well as decreased lipid peroxidation (LPO) content, were detected, especially in liver. Though the decrease in catalase activity indicated that the glycation compounds from HAHp(9.0)-G MRPs might be absorbed in vivo, the lower SOD/GPx ratios of HAHp(9.0)-G MRPs-fed groups than those of the normal groups also suggested that the administration of HAHp(9.0)-G MRPs could reduce LPO stress in mice. Our results suggest that a higher antioxidant status could be generated in the healthy animals after fed with HAHp(9.0)-G MRPs for 14 days.