Immune-mediated cholangiohepatitis in neonatally thymectomized mice: the role of T cells and analysis of T-cell receptor Vbeta usage.
Immune-mediated cholangiohepatitis in neonatally thymectomized mice: the role of T cells and analysis of T-cell receptor Vbeta usage.
复制标题
新生胸腺切除小鼠中免疫介导的胆管肝炎：T 细胞的作用和 T 细胞受体 Vbeta 使用分析。
作者：
Y. Aisaka;Y. Watanabe;M. Kamiyasu;T. Masanaga;K. Tsuji;T. Nakanishi;G. Kajiyama;M. Gershwin
We have previously reported the induction of immune-mediated cholangiohepatitis following injection of a hybrid recombinant proteins containing the E2 of the pyruvate dehydrogenase (PDC-E2) and the branched-chain keto-acid dehydrogenase (BCOADC-E2) to neonatally thymectomized (Tx) A/J mice. Further, we demonstrated that intrahepatic infiltrating mononuclear cells could transfer pathology to other Tx mice. To further our observations, we examined intrahepatic infiltrating mononuclear cells by flow cytometry and used cell transfer experiments to identify the phenotype involved. Interestingly, following immunization of neonatally Tx A/J mice and immunization with the bihybrid molecule, the number of CD3+infiltrating mononuclear cells were significantly higher (77.8%) compared with the control group. There was a small although not significant increase among intrahepatic infiltrating mononuclear cells and splenic cells of Vbeta 5.1,5.2+, Vbeta7+and Vbeta17+. In addition, Vbeta14+cells accounted for 20.4% of the infiltrating T-cells (P<0.01 vs. the control group). In further experiments, CD3+, CD4+or CD8+cells were isolated and removed from intrahepatic infiltrating mononuclear cells and subpopulations of mononuclear cells transferred to Tx mice. Both CD3+CD4+cells and CD3+CD8+cells are required for development of the lesion, and the damage is mediated by CD3+Vbeta14+cells.
登录
查看更多内容
MHC 多态性对 T 细胞识别细菌超抗原的不同影响。
DOI：
--
发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Wen,R;Blackman,MA;Woodland,DL
通讯作者：
Woodland,DL
免疫介导的胆管炎啮齿动物模型的开发和表征。
DOI：
10.1073/pnas.93.1.216
发表时间：
1996
影响因子：
11.1
作者：
Ueno,Y;Phillips,JO;Ludwig,J;Lichtman,SN;LaRusso,NF
通讯作者：
LaRusso,NF
新生胸腺切除小鼠免疫介导的胆管肝炎的诱导和持续。
DOI：
10.1006/clin.1998.4599
发表时间：
1998
期刊：
Clinical immunology and immunopathology
影响因子：
--
作者：
Masanaga,T;Watanabe,Y;VandeWater,J;Leung,PS;Nakanishi,T;Kajiyama,G;Ruebner,BH;Coppel,RL;Gershwin,ME
通讯作者：
Gershwin,ME
胸腺切除术第 3 天诱发的小鼠自身免疫性卵巢炎、附睾睾丸炎和胃炎。
DOI：
--
发表时间：
1987
期刊：
The American journal of pathology
影响因子：
--
作者：
Tung,KS;Smith,S;Matzner,P;Kasai,K;Oliver,J;Feuchter,F;Anderson,RE
通讯作者：
Anderson,RE