IMMUNOCYTOCHEMICAL LOCALIZATION OF THE CHEMOKINES RANTES AND MIP-1-ALPHA WITHIN HUMAN PLATELETS AND THEIR RELEASE DURING STORAGE
IMMUNOCYTOCHEMICAL LOCALIZATION OF THE CHEMOKINES RANTES AND MIP-1-ALPHA WITHIN HUMAN PLATELETS AND THEIR RELEASE DURING STORAGE
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DOI：
10.1159/000237097
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发表时间：
1995-08-01
影响因子：
2.8
通讯作者：
KUHNEL, W
中科院分区：
文献类型：
--
作者：
KLINGER, MHF;WILHELM, D;KUHNEL, W
细胞因子RANTES和MIP-1 α是属于趋化因子C-C亚家族的8-kD肽。它们都是嗜碱性粒细胞和嗜酸性粒细胞的强效趋化因子。除淋巴细胞外，最近显示RANTES肽从凝血酶刺激的人血小板中释放[Kameyoshi等：J Exp Med 1992;176：587-592]。采用植入后免疫细胞化学，我们可以检测RANTES和第一次也MIP-1 α内的人血小板的α-颗粒。迄今为止，未报告MIP-1 α在血小板中发生。在储存的血小板浓缩物（PC）中发现的轻度活化血小板中，标记RANTES和MIP-1 α。也可以在开放小管系统的顺式内和质膜上观察到，表明两种肽的释放。这些发现通过PC中的体外研究，通过研究RANTES和MIP-1 α释放到悬浮介质中得到证实。在8天的时间内，RANTES以相对高的量稳定释放，而MIP-1 α在悬浮培养基中以相当小的量测量。由于RANTES和MIP-1 α除了对嗜酸性粒细胞和嗜碱性粒细胞具有趋化活性外，还能够介导组胺的释放，因此很容易推测血小板参与了嗜酸性粒细胞和嗜碱性粒细胞参与的炎症反应。
The cytokines RANTES and MIP-1 alpha are 8-kD peptides which belong to the C-C subfamily of chemokines. They are both potent chemotactic factors for basophils and eosinophils. Apart from lymphocytes, the RANTES peptides was recently shown to be released from human platelets stimulated with thrombin [Kameyoshi et al: J Exp Med 1992;176:587-592]. Employing postembedding immunocytochemistry, we could detect RANTES and for the first time also MIP-1 alpha within the a-granules of human platelets. To date, MIP-1 alpha was not reported to occur in platelets. In slightly activated platelets, as found in stored platelet concentrates (PC), label for RANTES and MIP-1 alpha. could also be observed within cisterns of the open canalicular system and on the plasma membrane, indicating a release of both peptides. These findings were confirmed by in vitro studies in PC, by investigation of RANTES and MIP-1 alpha release into the suspending medium. Over a period of 8 days, RANTES was steadily released in relatively high amounts, whereas MIP-1 alpha was measured in rather small amounts in the suspending medium. As RANTES and MIP-1 alpha, besides their chemotactic activity on eosinophils and basophils, are able to mediate the release of histamine, it is tempting to speculate about a participation of platelets in inflammatory reactions in which eosinophils and basophils are involved.