Remobilization causes site-specific cyst formation in immobilization-induced knee cartilage degeneration in an immobilized rat model.
Remobilization causes site-specific cyst formation in immobilization-induced knee cartilage degeneration in an immobilized rat model.
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DOI：
10.1111/joa.12453
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发表时间：
2016-06
影响因子：
2.4
通讯作者：
Kuroki H
中科院分区：
文献类型：
--
作者：
Nagai M;Ito A;Tajino J;Iijima H;Yamaguchi S;Zhang X;Aoyama T;Kuroki H
了解关节软骨退变过程对于预防和治疗关节疾病是必要的。本研究旨在探讨长期固定引起的软骨退变是如何因重新活动而加剧的。本研究使用了 60 只 8 周大的雄性 Wistar 大鼠。使用外固定器固定单侧膝关节8 周。取出固定器后第0天和第3天以及第1周、第2周、第4周和第8周处死大鼠。处死大鼠后，测量最大膝关节伸展角度。制备内侧中髁区域（股骨和胫骨的非接触、过渡和接触区域）的组织学切片并评分。测量软骨厚度和软骨细胞数量，并通过免疫组织化学评估 CD44 和 Col2-3/4c 表达水平。组织学评估显示过渡区软骨退变逐渐加剧，软骨细胞和 CD44 阳性软骨细胞数量减少，并且随着时间的推移评分较差，特别是在胫骨中。复健后第 8 周，在胫骨过渡区域证实囊肿形成。过渡区的软骨厚度比接触区的软骨厚度厚，尤其是胫骨。在非接触区和过渡区观察到 Col2-3/4c 表达，膝关节伸展角度恢复。总之，随着时间的推移，过渡区域的再活动加剧了固定引起的软骨退变，随后观察到软骨细胞数量减少以及不同软骨区域之间的形态差异。
An understanding of the articular cartilage degenerative process is necessary for the prevention and treatment of joint disease. The present study aimed to examine how long‐term immobilization‐induced cartilage degeneration is aggravated by remobilization. Sixty 8‐week‐old male Wistar rats were used in this study. The unilateral knee joint was immobilized using an external fixator for 8 weeks. The rats were killed at 0 and 3 days, and at 1, 2, 4 and 8 weeks after removing the fixator. After the rats were killed, the maximum knee extension angles were measured. Histological sections at the medial mid‐condylar region (non‐contact, transitional and contact regions of the femur and tibia) were prepared and scored. The cartilage thickness and number of chondrocytes were measured, and CD44 and Col2‐3/4c expression levels were assessed immunohistochemically. The histological assessment revealed progressive aggravation of cartilage degeneration in the transitional region, with a decreased number of chondrocytes and CD44‐positive chondrocytes as well as poor scoring over time, particularly in the tibia. Cyst formation was confirmed in the transitional region of the tibia at 8 weeks post‐remobilization. The cartilage thickness in the transitional region was thicker than that in the contact region, particularly in the tibia. Col2‐3/4c expression was observed in the non‐contact and transitional regions, and the knee extension angle was recovered. In conclusion, immobilization‐induced cartilage degeneration was aggravated by remobilization over time in the transitional region, followed by observations of a decreased number of chondrocytes and morphological disparity between different cartilage regions.