Rapid transmission and tight bottlenecks constrain the evolution of highly transmissible SARS-CoV-2 variants.
Rapid transmission and tight bottlenecks constrain the evolution of highly transmissible SARS-CoV-2 variants.
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快速传播和严格的瓶颈限制了高传播性 SARS-CoV-2 变种的进化。
DOI：
10.1101/2022.10.12.511991
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Lauring,AdamS
中科院分区：
文献类型：
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作者：
Bendall,EmilyE;Callear,Amy;Getz,Amy;Goforth,Kendra;Edwards,Drew;Monto,ArnoldS;Martin,EmilyT;Lauring,AdamS
传播瓶颈限制了新突变的传播，并降低了沿着传播链的选择效率。虽然感染力增加、受体结合或免疫逃避可能会影响瓶颈大小，但传播性和传播瓶颈之间的关系尚不清楚。在这里，我们比较了非VOC SARS-CoV-2谱系与Alpha，Delta和Omicron的传播瓶颈。我们对来自65个家庭的168个人的病毒进行了测序。大多数病毒群体具有0-1个单核苷酸变体（iSNV）。从具有可检测iSNV的64个传输对中，我们确定了Alpha、Delta和Omicron的每个进化枝瓶颈为1（95%CI 1-1），非VOC的每个进化枝瓶颈为2（95%CI 2-2）。这些紧密的瓶颈反映了传播时的低多样性，这在快速传播的变体中可能更明显。紧密的瓶颈将限制高度突变的VOC在传播链中的发展，这进一步证明选择而不是长期感染可能会推动它们的进化。
Transmission bottlenecks limit the spread of novel mutations and reduce the efficiency of selection along a transmission chain. While increased force of infection, receptor binding, or immune evasion may influence bottleneck size, the relationship between transmissibility and the transmission bottleneck is unclear. Here we compare the transmission bottleneck of non-VOC SARS-CoV-2 lineages to those of Alpha, Delta, and Omicron. We sequenced viruses from 168 individuals in 65 households. Most virus populations had 0–1 single nucleotide variants (iSNV). From 64 transmission pairs with detectable iSNV, we identify a per clade bottleneck of 1 (95% CI 1–1) for Alpha, Delta, and Omicron and 2 (95% CI 2–2) for non-VOC. These tight bottlenecks reflect the low diversity at the time of transmission, which may be more pronounced in rapidly transmissible variants. Tight bottlenecks will limit the development of highly mutated VOC in transmission chains, adding to the evidence that selection over prolonged infections may drive their evolution.