Cyclooxygenase-2 protein and prostaglandin E(2) production are up-regulated in a rat bladder inflammation model.
Cyclooxygenase-2 protein and prostaglandin E(2) production are up-regulated in a rat bladder inflammation model.
复制标题
在大鼠膀胱炎症模型中，Cyclooxygenase-2 蛋白和前列腺素 E(2) 的产生上调。
DOI：
10.1016/s0014-2999(01)00911-6
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发表时间：
2001
影响因子：
5
通讯作者：
Weiss,RM
中科院分区：
文献类型：
--
作者：
Wheeler,MA;Yoon,JH;Olsson,LE;Weiss,RM
Cyclooxygenase-1 and cyclooxygenase-2 mRNAs and proteins and prostaglandin E2production are evaluated in a rat model of inflammation in which Escherichia coli lipopolysaccharide is intraperitoneally injected or intravesically instilled into the bladder. While cyclooxygenase-1 mRNA and protein and cyclooxygenase-2 mRNA do not change in bladders treated with lipopolysaccharide, cyclooxygenase-2 protein is elevated in bladders from rats intravesically instilled with lipopolysaccharide or phosphate buffered saline (PBS) or intraperitoneally injected with lipopolysaccharide. Urinary prostaglandin E2levels and prostaglandin E2synthesis in bladder particulates are elevated by intravesical instillation and intraperitoneal injection of lipopolysaccharide. The nitric oxide donor, S-nitroso-N-acetyl-d,l-penicillamine, increases prostaglandin E2synthesis in bladders from lipopolysaccharide intravesically instilled and intraperitoneally injected rats. Lipopolysaccharide increases prostaglandin E2synthesis by increasing cyclooxygenase-2 protein levels in rat bladder and prostaglandin E2synthesis may be further elevated by increases in nitric oxide caused by an up-regulation of inducible nitric oxide synthase (iNOS).
