Characterization and complete genomic analysis of two Salmonella phages, SenALZ1 and SenASZ3, new members of the genus Cba120virus
Characterization and complete genomic analysis of two Salmonella phages, SenALZ1 and SenASZ3, new members of the genus Cba120virus
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DOI：
10.1007/s00705-019-04183-3
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发表时间：
2019-05-01
影响因子：
2.7
通讯作者：
Fu, Xiongfei
中科院分区：
文献类型：
--
作者：
Chen, Ling;Guan, Guoye;Fu, Xiongfei
分离并分析了两种新的沙门氏菌SenALZ 1和SenASZ 3。这两个品种的基因组分别由154，811和157，630个碱基组成，G+C含量分别为44.56%和44.74%。SenALZ 1基因组中199个开放阅读框（ORF）中的59个和SenASZ 3基因组中204个中的60个显示与NCBI nr数据库中编码具有预测功能的推定噬菌体蛋白的参考序列相似。根据透射电镜（TEM）观察、全基因组序列比对、系统发育分析和基因注释，我们认为这两株病毒是阿克曼病毒科Cvivirinae亚科Cba 120病毒属的两个新种的代表性分离物。
Salmonella phages SenALZ1 and SenASZ3, two novel phages infecting Salmonella enterica, were isolated and analyzed. The genomes of these two phages consist of 154,811 and 157,630 base pairs (bp), with G+C contents of 44.56% and 44.74%, respectively. Fifty-nine of 199 open reading frames (ORFs) in the SenALZ1 genome, and 60 of the 204 in the SenASZ3 genome show similarity to reference sequences in the NCBI nr database that encode putative phage proteins with predicted functions. Based on the results of transmission electron microscopy (TEM) examination, complete genome sequence alignment, phylogenetic analysis, and gene annotation, we propose that these two phages are representative isolates of two new species of the genus Cba120virus, subfamily Cvivirinae, family Ackermannviridae.