ISOLATION, STRUCTURE, AND EXPRESSION OF LABIAL, A HOMEOTIC GENE OF THE ANTENNAPEDIA COMPLEX INVOLVED IN DROSOPHILA HEAD DEVELOPMENT
ISOLATION, STRUCTURE, AND EXPRESSION OF LABIAL, A HOMEOTIC GENE OF THE ANTENNAPEDIA COMPLEX INVOLVED IN DROSOPHILA HEAD DEVELOPMENT
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DOI：
10.1101/gad.3.3.399
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发表时间：
1989-03-01
影响因子：
10.5
通讯作者：
KAUFMAN, TC
中科院分区：
文献类型：
--
作者：
DIEDERICH, RJ;MERRILL, VKL;KAUFMAN, TC
果蝇的唇基因（la）是胚胎（幼虫）和成虫头部正常发育所必需的。我们通过绘制与染色体重排的实验室等位基因相关的分子病变，确定了在近端部分的内的实验室转录单位的napedia复合体（ANT-C）。我们目前在分子结构，核苷酸序列，和时间模式的表达。此外，使用针对融合蛋白产生的抗体，我们表明，在胚胎中的实验室蛋白分布在神经和表皮细胞的procephalic叶;在一个离散的循环中肠;和特定的祖感觉细胞的clypeolabrum，胸段，和尾部区域。发育中的脑中的实验室表达区域代表了非分段结构域，其在变形（Dfd）和长鼻足（pb）基因的表达结构域之前并且在很大程度上与变形（Dfd）和长鼻足（pb）基因的表达结构域不重叠，变形（Dfd）和长鼻足（pb）基因是ANT-C的另外两个同源异型基因座，其也起到指导头部结构发育的作用。此外，实验室头部表达与头部退化的复杂细胞运动相关，这一过程不仅在实验室胚胎中有缺陷，而且其失败似乎是突变胚胎中观察到的缺陷的主要原因。最后，我们认为，实验室头的表达provdies的一个分子标记的一个intercalary段，祖先的部分，已成为形态上模糊的昆虫头部的进化过程中。
The labial (la) gene of Drosophila melanogaster is necessary for the proper development of the embryonic (larval) and adult head. We have identified the lab transcription unit within the proximal portion of the Antennapedia Complex (ANT-C) by mapping the molecular lesions associated with chromosomally rearranged lab alleles. We present in molecular structure, nucleotide sequence, and temporal pattern of expression. In addition, using antibodies generated against a fusion protein, we show that in the embryo the lab protein is distributed in neural and epidermal cells of the procephalic lobe; in a discrete loop of the midgut; and in specific progenitor sensory cells of the clypeolabrum, thoracic segments, and tail region. The regions of lab expression in the developing cephalon represent nonsegmented domains that are anterior to and largely nonoverlapping with the domains of expression of the Deformed (Dfd) and proboscipedia (pb) genes, two other homeotic loci of the ANT-C that also function to direct the development of head structures. Furthermore, lab head expression is associated with the complex cellular movement of head involution, a process that not only is defective in lab- embryos, but the failure of which appears to be largely responsible for the defects observed in mutant embryos. Finally, we suggest that lab head expression provdies a molecular marker for an intercalary segment, an ancestral segment that has become morphologically indistinct during the evolution of the insect head.