MR elastography in a murine stroke model reveals correlation of macroscopic viscoelastic properties of the brain with neuronal density
MR elastography in a murine stroke model reveals correlation of macroscopic viscoelastic properties of the brain with neuronal density
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DOI：
10.1002/nbm.2987
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发表时间：
2013-11-01
影响因子：
2.9
通讯作者：
Braun, Juergen
中科院分区：
文献类型：
--
作者：
Freimann, Florian Baptist;Mueller, Susanne;Braun, Juergen
本研究的目的是利用磁共振弹性成像（MRE）研究神经元密度对小鼠缺血性梗塞后活体脑组织粘弹性参数的影响。在 20 只小鼠中诱导左半球短暂大脑中动脉闭塞 (MCAO)。 MCAO后第3、7、14和28天（每组n = 5）进行体内7-T MRE，振动频率为900 Hz，然后分析组织学标记，例如神经元计数（NeuN）。 MCAO 导致左半球的储能模量相对于对侧值显着降低 (p=0.03)，且不随时间变化。观察到两个半球的储能模量和 NeuN 之间存在相关性，相关系数为 R=0.648（p=0.002，左）和 R=0.622（p=0.003，右）。损耗模量对 MCAO 不太敏感，但与左半球的 NeuN 相关（R=0.764，p=0.0001）。与文献一致，这些结果表明短暂性脑缺血损伤后大脑中的剪切模量降低。此外，我们的研究提供了证据表明脑组织的体内剪切模量与神经元密度相关。因此，在诊断应用中，MRE 作为基于图像的神经退行性过程量化工具可能具有诊断潜力。版权所有 (c) 2013 John Wiley & Sons, Ltd.
The aim of this study was to investigate the influence of neuronal density on viscoelastic parameters of living brain tissue after ischemic infarction in the mouse using MR elastography (MRE). Transient middle cerebral artery occlusion (MCAO) in the left hemisphere was induced in 20 mice. In vivo 7-T MRE at a vibration frequency of 900 Hz was performed on days 3, 7, 14 and 28 (n=5 per group) after MCAO, followed by the analysis of histological markers, such as neuron counts (NeuN). MCAO led to a significant reduction in the storage modulus in the left hemisphere relative to contralateral values (p=0.03) without changes over time. A correlation between storage modulus and NeuN in both hemispheres was observed, with correlation coefficients of R=0.648 (p=0.002, left) and R=0.622 (p=0.003, right). The loss modulus was less sensitive to MCAO, but correlated with NeuN in the left hemisphere (R=0.764, p=0.0001). In agreement with the literature, these results suggest that the shear modulus in the brain is reduced after transient ischemic insult. Furthermore, our study provides evidence that the in vivo shear modulus of brain tissue correlates with neuronal density. In diagnostic applications, MRE may thus have diagnostic potential as a tool for image-based quantification of neurodegenerative processes. Copyright (c) 2013 John Wiley & Sons, Ltd.