Reduced expression of CTR1 gene modulated by mitochondria causes enhanced ethylene response in cytoplasmic male-sterile Brassica juncea
Reduced expression of CTR1 gene modulated by mitochondria causes enhanced ethylene response in cytoplasmic male-sterile Brassica juncea
复制标题
线粒体调节的CTR1基因表达减少导致细胞质雄性不育芥菜乙烯反应增强
DOI：
10.1111/j.1399-3054.2012.01588.x
复制
发表时间：
2012-06-01
影响因子：
6.4
通讯作者：
Zhang, Mingfang
中科院分区：
文献类型：
--
作者：
Liu, Xunyan;Yang, Xiaodong;Zhang, Mingfang
我们研究了线粒体如何通过调控CTR1表达影响芥菜细胞质雄性不育（CMS）对乙烯的反应。与雄性可育系相比，CMS中CTR1基因的表达降低。我们观察到，在黑暗条件下萌发时，下胚轴和根的长度比MF系短。经1-氨基环丙烷-1-羧酸处理后，CMS植株对乙烯的响应增强。经Ag+处理后，CMS植株的表型可恢复到保持系。在CMS中发现了一个乙烯应答基因，即植物防御素基因。这种表型行为可以通过干扰线粒体功能的抗霉素A处理维持系来模拟，显示下胚轴和根的长度减少，导致乙烯相关基因的表达模式与CMS相似。用赤霉素（GA3）处理后，下胚轴和根的长度可以恢复到维持系。此外，经抗霉素a处理的CMS植株和MF植株中GA3含量降低，乙烯处理对GA3含量影响显著；而GA3对乙烯调控下胚轴和根长相关基因的表达影响不显著，说明乙烯可能通过赤霉素上游调控下胚轴和根的发育。综上所述，我们的研究结果表明，乙烯和赤霉素途径的线粒体调节与调节下胚轴和根的发育之间存在联系。
We studied how mitochondria affect ethylene response via modulation of CTR1 expression in cytoplasmic male-sterile (CMS) Brassica juncea. The expression of CTR1 gene was reduced in CMS compared with male-fertile (MF) lines. We observed that hypocotyl and root lengths were shorter than in the MF line during germination in the dark. An enhanced ethylene response was observed in CMS plants as shown by the CMS and maintainer line phenotypes treated with 1-aminocyclopropane-1-carboxylic acid. The phenotype in CMS plants could be recovered to the maintainer line when treated with Ag+. One ethylene response gene, plant defensin gene, was detected to be induced in CMS. The behavior of this phenotype could be mimicked by treating the maintainer line with antimycin A that disturbs mitochondrial function, which showed reduced length of hypocotyl and roots, and resulted in similar expression patterns of ethylene-related genes as in CMS. The reduced length of hypocotyl and roots could be recovered to the maintainer line by treatment with gibberellic acid (GA3). In addition, the GA3 content was reduced in CMS plants and in the MF line treated with antimycin A. Ethylene treatment markedly affects GA3 content; however, GA3 did not significantly affect ethylene-related gene expression in regards to regulation of hypocotyl and root length, which suggests that ethylene acts upstream via gibberellin to regulate hypocotyls and root development. Taken together, our results suggest a link between mitochondrial modulation of the ethylene and gibberellin pathway that regulates the development of hypocotyl and roots.