Functional characterization of an LCCL-lectin domain containing protein family in Plasmodium berghei
Functional characterization of an LCCL-lectin domain containing protein family in Plasmodium berghei
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DOI：
10.1645/ge-3368
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发表时间：
2004-10-01
影响因子：
1.3
通讯作者：
Sinden, RE
中科院分区：
文献类型：
--
作者：
Trueman, HE;Raine, JD;Sinden, RE
使用生物信息学、蛋白质组学、免疫荧光和遗传交叉方法，我们对一系列假定的寄生虫配体进行了功能表征，作为细胞间相互作用的潜在介质。我们将这些蛋白质命名为鲎凝血因子 C、Coch-5b2 和 Lg11 (LCCL)-凝集素粘附样蛋白 (LAP) 家族。我们证明这个家族在疟原虫属中是保守的。它具有通常与细胞外蛋白相关的粘附蛋白结构域的独特排列。这些蛋白质主要（但不完全）在生命周期的蚊子阶段表达。我们测试了这些蛋白是表面蛋白的假设，其中 I 是该基因家族的成员 lap1，并提供了它在伯氏疟原虫子孢子表面表达的证据。最后，通过野生型Pblap1（+）和转基因Pblap1（-）寄生虫的遗传杂交，我们表明先前报道的Pblap1（-）突变体子孢子发育的无效表型可以在异核卵囊中得到挽救，并且可以形成感染性Pblap1（-）子孢子。蚊子与 Pblap1(+) 和 Pblap1(-) 同核卵囊混合物的共寄生不能拯救该突变体。
Using bioinformatic, proteomic, immunofluorescence, and genetic cross methods, we have functionally characterized a family of putative parasite ligands as potential mediators of cell-cell interactions. We name these proteins the Limulus clotting factor C, Coch-5b2, and Lg11 (LCCL)-lectin adhesive-like protein (LAP) family. We demonstrate that this family is conserved amongst Plasmodium spp. It possesses a unique arrangement of adhesive protein domains normally associated with extracellular proteins. The proteins are expressed predominantly, though not exclusively, in the mosquito stages of the life cycle. We test the hypothesis that these proteins are surface proteins with I member of this gene family, lap1, and provide evidence that it is expressed on the surface of Plasmodium berghei sporozoites. Finally, through genetic crosses of wild-type Pblap1(+) and transgenic Pblap1(-) parasites, we show that the null phenotype previously reported for sporozoite development in a Pblap1(-) mutant can be rescued within a heterokaryotic oocyst and that infectious Pblap1(-) sporozoites can be formed. The mutant is not rescued by coparasitization of mosquitoes with a mixture Pblap1(+) and Pblap1(-) homokaryotic oocysts.