Expanding Role of the Jumonji C Domain as an RNA Hydroxylase
Expanding Role of the Jumonji C Domain as an RNA Hydroxylase
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DOI：
10.1074/jbc.m110.156398
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发表时间：
2010-11-05
影响因子：
4.8
通讯作者：
Suzuki, Tsutomu
中科院分区：
文献类型：
--
作者：
Noma, Akiko;Ishitani, Ryuichiro;Suzuki, Tsutomu
JmjC(Jumonji C)结构域蛋白是一个广泛的Fe(II)/2-氧代戊二酸依赖的加氧酶家族，通过催化甲基化组蛋白的氧化脱甲基化参与基因表达的表观遗传调控。我们在这里报道了一个名为Tyw5p(TYW5)的人JmjC蛋白通过催化羟化作用，在tRNA(Phe)中意外地作用于一种高度修饰的核苷--羟基乳清蛋白的生物合成。这一发现为JmjC蛋白作为RNA羟基酶的扩展作用提供了洞察力。
JmjC (Jumonji C) domain-containing proteins are known to be an extensive family of Fe(II)/2-oxoglutarate-dependent oxygenases involved in epigenetic regulation of gene expression by catalyzing oxidative demethylation of methylated histones. We report here that a human JmjC protein named Tyw5p (TYW5) unexpectedly acts in the biosynthesis of a hypermodified nucleoside, hydroxywybutosine, in tRNA(Phe) by catalyzing hydroxylation. The finding provides an insight into the expanding role of JmjC protein as an RNA hydroxylase.