Single-dose ebselen does not afford sustained neuroprotection to rats subjected to severe focal cerebral ischemia
Single-dose ebselen does not afford sustained neuroprotection to rats subjected to severe focal cerebral ischemia
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DOI：
10.1016/j.ejphar.2004.05.024
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发表时间：
2004-07-08
影响因子：
5
通讯作者：
Alborch, E
中科院分区：
文献类型：
--
作者：
Salom, JB;Pérez-Asensio, FJ;Alborch, E
Oxygen free radicals have been involved in the pathophysiology of cerebral ischemia, especially after spontaneous or thrombolytic reperfusion. In this study with rats, we have combined a severe focal ischemic insult (2 h) and a prolonged reperfusion time (7 days) to assess the possible sustained neuroprotective effect of ebselen (10 or 100 mg/kg), a small, lipophilic organoselenium compound which mimics glutathione peroxidase. Parietal cortical perfusion was measured by laser-Doppler flowmetry, and focal cerebral ischemia was carried out by the intraluminal thread method. We have measured plasma selenium levels, brain reduced glutathione levels, as a marker of oxidative stress, and infarct volume associated with cerebral ischemia. Focal ischemia did not alter reduced glutathione levels, while 60 min reperfusion following ischemia induced a significant (P
