Genetic diversity and population structure of the roughskin sculpin (Trachidermus fasciatus Heckel) inferred from microsatellite analyses: implications for its conservation and management
Genetic diversity and population structure of the roughskin sculpin (Trachidermus fasciatus Heckel) inferred from microsatellite analyses: implications for its conservation and management
复制标题
从微卫星分析推断粗皮杜父鱼（Trachidermus fasciatus Heckel）的遗传多样性和种群结构：对其保护和管理的影响
DOI：
10.1007/s10592-016-0832-7
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发表时间：
2016-08-01
影响因子：
2.2
通讯作者：
Liu, Jin-Xian
中科院分区：
文献类型：
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作者：
Li, Yu-Long;Xue, Dong-Xiu;Liu, Jin-Xian
种群单元的识别对于管理和监测方案至关重要，特别是对于濒危野生物种。粗皮杜父鱼（Trachidermus fasciatus Heckel）是一种小型降河产卵鱼类，1988年被列为国家二级保护水生动物。为了实现该物种的可持续保护，有必要澄清种群之间和种群内现有的遗传结构。在此，我们对8个群体的遗传结构进行了研究。利用16个高度多态性的微卫星标记对10个江豚的遗传多样性进行了分析。在所有人群中观察到高水平的遗传变异。在错误发现率校正后，所有成对FST估计值均具有显著性（总体平均FST = 0.054）。此外，结构和主成分判别分析（DAPC）分析表明，8个群体分为6个集群。BAYESASS分析表明，人口之间最近的移徙普遍较低，而且不对称。所有这些结果表明，显着的遗传结构在种群之间。然而，没有隔离的距离关系的种群，可能是由于栖息地破碎化所造成的基因流的障碍。我们的研究结果强调需要在原地保护工作的T。因此，我们可以通过最大化栖息地的大小和质量，在整个分布范围内，保护整体的遗传多样性和进化潜力。
Identification of population units is crucial for management and monitoring programs, especially for endangered wild species. The roughskin sculpin (Trachidermus fasciatus Heckel) is a small catadromous fish and has been listed as a second class state protected aquatic animal since 1988 in China. To achieve sustainable conservation of this species, it is necessary to clarify the existing genetic structure both between and within populations. Here, population genetic structure among eight populations of T. fasciatus were analyzed by using 16 highly polymorphic microsatellites. High levels of genetic variation were observed in all populations. All pairwise FST estimates were significant after false discovery rate correction (overall average FST = 0.054). Furthermore, both STRUCTURE and discriminant analysis of principal components (DAPC) analysis showed that the eight populations were grouped into six clusters. BAYESASS analysis showed generally low recent and asymmetric migration among populations. All these results suggested significant genetic structure across populations. However, there was no isolation by distance relationship among populations, likely resulting from barriers to gene flow created by habitat fragmentation. Our results highlight the need for in situ conservation efforts for T. fasciatus across its entire distribution range, through maximizing habitat size and quality to preserve overall genetic diversity and evolutionary potential.