Identification of QTLs Conferring Resistance to Deltamethrin in Culex pipiens pallens.
Identification of QTLs Conferring Resistance to Deltamethrin in Culex pipiens pallens.
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DOI：
10.1371/journal.pone.0140923
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yan G
中科院分区：
文献类型：
--
作者：
Zou F;Chen C;Zhong D;Shen B;Zhang D;Guo Q;Wang W;Yu J;Lv Y;Lei Z;Ma K;Ma L;Zhu C;Yan G
淡色库蚊（Culex pipiens pallens）是我国北方分布最丰富的库蚊种，是班氏丝虫病和西尼罗病毒的重要传播媒介。溴氰菊酯是一种广泛用于蚊虫控制的杀虫剂，但对该杀虫剂和其他杀虫剂的耐药性已成为控制似乎具有定量遗传性的病媒传播疾病的主要挑战。此外，对杀虫剂抗药性的遗传基础仍然知之甚少。采用混合分离分析（BSA）和扩增片段长度多态性标记（AFLP）技术，对淡色库蚊（Culex pipiens pallens）的溴氰菊酯抗性进行了QTL定位。构建了一张覆盖381 cM的遗传连锁图，通过复合区间作图（CIM）共检测到7个抗性QTL，解释了95%的表型变异。连锁群2中的主效QTL解释了62%的变异，值得进一步研究。对图谱中的12个AFLP标记进行了克隆，并利用BLAST工具对近缘致倦库蚊（Culex quinquefasciatus）基因组序列进行了定位。结果表明，淡色库蚊对溴氰菊酯的抗性是一个受主效QTL控制的数量性状。相关AFLP标记的克隆证实了将遗传图谱锚定到物理图谱的潜在效用。这些结果提供了对性状遗传结构的深入了解。
Culex pipiens pallens is the most abundant Culex mosquito species in northern China and is an important vector of bancroftian filariasis and West Nile virus. Deltamethrin is an insecticide that is widely used for mosquito control, however resistance to this and other insecticides has become a major challenge in the control of vector-borne diseases that appear to be inherited quantitatively. Furthermore, the genetic basis of insecticide resistance remains poorly understood. In this study, quantitative trait loci (QTL) mapping of resistance to deltamethrin was conducted in F2 intercross segregation populations using bulked segregation analysis (BSA) and amplified fragment length polymorphism markers (AFLP) in Culex pipiens pallens. A genetic linkage map covering 381 cM was constructed and a total of seven QTL responsible for resistance to deltamethrin were detected by composite interval mapping (CIM), which explained 95% of the phenotypic variance. The major QTL in linkage group 2 accounted for 62% of the variance and is worthy of further study. 12 AFLP markers in the map were cloned and the genomic locations of these marker sequences were determined by applying the Basic Local Alignment Search Tool (BLAST) tool to the genome sequence of the closely related Culex quinquefasciatus. Our results suggest that resistance to deltamethrin is a quantitative trait under the control of a major QTL in Culex pipiens pallens. Cloning of related AFLP markers confirm the potential utility for anchoring the genetic map to the physical map. The results provide insight into the genetic architecture of the trait.