Isoliquiritigenin as an antioxidant phytochemical ameliorates the developmental anomalies of zebrafish induced by 2,2 ',4,4 '-tetrabromodiphenyl ether
Isoliquiritigenin as an antioxidant phytochemical ameliorates the developmental anomalies of zebrafish induced by 2,2 ',4,4 '-tetrabromodiphenyl ether
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异甘草素作为抗氧化剂植物化学物质可改善 2,2',4,4'-四溴二苯醚引起的斑马鱼发育异常
DOI：
10.1016/j.scitotenv.2019.02.272
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发表时间：
2019
影响因子：
9.8
通讯作者：
Wang Shou-Lin
中科院分区：
文献类型：
--
作者：
Wang Chao;Yang Lu;Hu Yuhuan;Zhu Jiansheng;Xia Rong;Yu Yongquan;Shen Jiemiao;Zhang Zhan;Wang Shou-Lin
2，2 ′，4，4 ′-四溴二苯醚（BDE 47）是生物样品中含量最丰富的多溴二苯醚同系物。它具有很强的生物累积倾向，并通过氧化应激可能危及哺乳动物的发育。异甘草素（Isoliquiritigenin，ISL）是一种新兴的天然查尔酮类黄酮化合物，具有抗氧化、抗过敏、抗炎、抗肿瘤和雌激素活性等多种生物学和药理学活性。本研究的目的是探讨ISL对BDE 47所致发育异常的改善作用。斑马鱼（Danio rerio）胚胎在受精后（hpf）暴露于BDE 47（1和10 μM）和/或ISL（4 μM）4 ~ 120 h，观察胚胎的形态、发育、行为、氧化应激状态和相关基因表达。结果表明，BDE-47可导致依赖剂量的生长迟缓和畸形，包括孵化延迟、脊柱弯曲、体长缩短、死亡率增加、行为异常和暗适应视力受损，ISL可显著缓解这些情况。此外，ISL还能抑制BDE 47诱导的斑马鱼体内ROS的过度积累，并上调凋亡相关基因p53、Bcl-2、caspase 3和caspase 9的表达，提示ISL通过抑制程序性凋亡和激活抗氧化信号通路，保护斑马鱼免受BDE 47的发育毒性。总而言之，开发ISL作为膳食补充剂可能是改善环境污染物诱导的发育毒性的一种有前途的预防策略。
2,2′,4,4′-Tetrabromodiphenyl ether (BDE47) is the most abundant PBDE congeners in biological samples. It has strong tendencies to bioaccumulate and potentially endangers development of mammals through oxidative stress. Isoliquiritigenin (ISL), an emerging natural chalcone-type flavonoid, possesses various biological and pharmacological properties, including antioxidant, anti-allergic, anti-inflammatory, anti-tumor and estrogenic activities. The purpose of the study is to explore the antioxidant effect of ISL on the amelioration of developmental anomalies induced by BDE47. Zebrafish (Danio rerio) embryos were exposed to BDE47 (1 and 10 μM) and/or ISL (4 μM) for 4 to 120 hours post fertilization (hpf), and the morphology, development, behavior, oxidative stress status and related genes expression were assessed. The results showed that BDE47 contributed to dose-dependent growth retardation and deformities, including delayed hatching, spinal curvature, reduced body length, increased death rate, aberrant behaviors and impaired dark-adapted vision, which were significantly mitigated by ISL. Besides, ISL ameliorated excessive ROS accumulation, and exaggerated the expressions of apoptosis-related genesp53,Bcl-2,caspase 3andcaspase 9induced by BDE47, suggesting that ISL protected zebrafish from the developmental toxicity of BDE47 by inactivation of programmed apoptosis and activation of antioxidant signaling pathways. Taken together, developing ISL as a dietary supplement might be a promising preventive strategy for the amelioration of developmental toxicity induced by environmental pollutants.