Comparison of cytotoxic extracts from fruiting bodies, infected insects and cultured mycelia of Cordyceps formosana.
Comparison of cytotoxic extracts from fruiting bodies, infected insects and cultured mycelia of Cordyceps formosana.
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DOI：
10.1016/j.foodchem.2013.09.001
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发表时间：
2014-02
期刊：
影响因子：
8.8
通讯作者：
Ruili Lu;Guan‐Hu Bao;Fenglin Hu;Bo Huang;Chun-ru Li;Zeng-zhi Li
中科院分区：
文献类型：
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作者：
Ruili Lu;Guan‐Hu Bao;Fenglin Hu;Bo Huang;Chun-ru Li;Zeng-zhi Li
A resazurin method was employed to test and compare cytotoxicity of extracts from fruiting bodies, insects and cultured mycelia ofCordyceps formosanaagainst Chinese hamster ovary (CHO) cells. Results showed that the cultured mycelia had much stronger cytotoxicity than that of the fruiting bodies and infected insects. This suggestes that using cultured mycelia to substitute a naturalCordycepsmay result in poisoning. A combined method of HPLC-PAD–HRMS and cytotoxic analysis revealed that the most toxic compound (Compound1) was found mainly in the cultured mycelia and also a small amount in the infected insect body of theCordyceps, but not in the fruiting body. The second toxic compound (Compound2) was found in all structures ofCordycepsand in cultured mycelia. Different contents of the toxic compounds resulted in the different cytotoxicity of the extracts. Compound1and Compound2were prepared with preparative HPLC as yellow and orange powders, respectively. Cytotoxic tests showed that the median lethal dose (LD50) against CHO cells of Compound1was 18.3 ± 0.2 and 103.7 ± 5.9 μg/mL for Compound2. Compound1and Compound2were identified as rugulosin and skyrin by HRMS, UV and NMR data. The two compounds were never previously isolated from the generaCordycepsandHirsutellaand their cytotoxicity against CHO cells was also reported for the first time.
