Reproducibility of urinary biomarkers in multiple 24-h urine samples
Reproducibility of urinary biomarkers in multiple 24-h urine samples
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DOI：
10.3945/ajcn.116.139758
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发表时间：
2017-01-01
影响因子：
7.1
通讯作者：
Willett, Walter C.
中科院分区：
文献类型：
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作者：
Sun, Qi;Bertrand, Kimberly A.;Willett, Walter C.
背景资料：关于24小时尿液样本中生物标志物的可重复性的有限知识阻碍了样本在流行病学研究中的收集和使用。目的：我们旨在评估重复24小时尿液样本中各种标志物的可重复性。设计：我们计算了在NHS的3168名参与者中收集的24小时尿液样本中测量的生物标志物的组内相关系数（ICC）。结果：在742名妇女中，在1年的时间内，每个收集4个样本，钠的ICC在NHS中为0.32，在NHSII中为0.34。在2439名男性和女性中，在1周至>= 1个月期间各采集2份样本，在不同的采集间隔期间，钠的ICC范围为0.33至0.68。尿中钾、钙、镁、磷酸盐、硫酸盐和其他尿标志物的排泄量通常显示出较高的重现性（ICC>0.4）。在47名妇女的两个24小时的尿液样本，ICC范围从0.15（儿茶素）到0.75（肠内酯）的多酚代谢物。对于邻苯二甲酸酯，ICC通常
Background: Limited knowledge regarding the reproducibility of biomarkers in 24-h urine samples has hindered the collection and use of the samples in epidemiologic studies.Objective: We aimed to evaluate the reproducibility of various markers in repeat 24-h urine samples.Design: We calculated intraclass correlation coefficients (ICCs) of biomarkers measured in 24-h urine samples that were collected in 3168 participants in the NHS (Nurses' Health Study), NHSII (Nurses' Health Study II), and Health Professionals Follow-Up Study.Results: In 742 women with 4 samples each collected over the course of 1 y, ICCs for sodium were 0.32 in the NHS and 0.34 in the NHSII. In 2439 men and women with 2 samples each collected over 1 wk to >= 1 mo, the ICCs ranged from 0.33 to 0.68 for sodium at various intervals between collections. The urinary excretion of potassium, calcium, magnesium, phosphate, sulfate, and other urinary markers showed generally higher reproducibility (ICCs >0.4). In 47 women with two 24-h urine samples, ICCs ranged from 0.15 (catechin) to 0.75 (enterolactone) for polyphenol metabolites. For phthalates, ICCs were generally