Cell death in a harmful algal bloom causing species Alexandrium tamarense upon an algicidal bacterium induction

Cell death in a harmful algal bloom causing species Alexandrium tamarense upon an algicidal bacterium induction
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杀藻细菌诱导后,有害藻华中的细胞死亡导致塔玛亚历山大藻物种死亡

DOI:
10.1007/s00253-014-5886-1
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发表时间:
2014-06
影响因子:
5
通讯作者:
Zheng Tianling
Zheng Tianling
中科院分区:
工程技术2区
文献类型:
--
作者:
Zhang Huajun;Lv Jinglin;Peng Yun;Zhang Su;An Xinli;Xu Hong;Zhang Jun;Tian Yun;Zheng Wei;Zheng Tianling

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有害藻华在世界各地发生,破坏水生生态系统并威胁人类健康。海洋杀藻细菌DHQ25的培养上清液能够裂解甲藻塔玛亚历山大藻。在细菌上清液胁迫下,检测到塔玛曲霉中光合色素的损失,并伴随着光系统 II (PSII) 光化学效率 (Fv/Fm) 的下降。透射电子显微镜分析显示,作为杀藻过程的一部分,叶绿体分解发生了明显的形态变化。 PSII电子传递链严重受阻,反应中心受损。这种损伤是在 psbA 和 psbD 基因的相对转录水平中检测到的,这些基因编码 PSII 反应中心的 D1 和 D2 蛋白。 PSII电子传递链中的阻断可能会产生过量的活性氧(ROS),从而破坏膜系统和色素合成以及激活酶抗氧化系统,包括超氧化物歧化酶(SOD)和过氧化氢酶(CAT)。这项研究表明,具有间接杀藻活性的海洋细菌在有害藻华物种光合作用过程的变化中发挥着重要作用。
Harmful algal blooms occur throughout the world, destroying aquatic ecosystems and threatening human health. The culture supernatant of the marine algicidal bacteria DHQ25 was able to lysis dinoflagellate Alexandrium tamarense. Loss of photosynthetic pigments, accompanied by a decline in Photosystem II (PSII) photochemical efficiency (Fv/Fm), in A. tamarense was detected under bacterial supernatant stress. Transmission electron microscope analysis showed obvious morphological modifications of chloroplast dismantling as a part of the algicidal process. The PSII electron transport chain was seriously blocked, with its reaction center damaged. This damage was detected in a relative transcriptional level of psbA and psbD genes, which encode the D1 and D2 proteins in the PSII reaction center. And the block in the electron transport chain of PSII might generate excessive reactive oxygen species (ROS) which could destroy the membrane system and pigment synthesis and activated enzymic antioxidant systems including superoxide dismutase (SOD) and catalase (CAT). This study indicated that marine bacteria with indirect algicidal activity played an important role in the changes of photosynthetic process in a harmful algal bloom species.
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