Control of cerebral cortical blood flow by stimulation of basal forebrain cholinergic areas in mice
Control of cerebral cortical blood flow by stimulation of basal forebrain cholinergic areas in mice
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DOI：
10.1007/s12576-011-0139-x
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发表时间：
2011-05-01
影响因子：
2.3
通讯作者：
Maruyama, Naoki
中科院分区：
文献类型：
--
作者：
Hotta, Harumi;Uchida, Sae;Maruyama, Naoki
我们用激光散斑和激光多普勒血流仪检测了Meynert基底核(NBM)的活动是否调节了小鼠大脑皮质局部血流量(RCBF)。在麻醉小鼠中，无论是电刺激还是化学刺激，一侧NBM的局部刺激都会增加同侧大脑皮质额叶、顶叶和枕叶的rCBF，而与全身血压的变化无关。大多数对低强度刺激(阈值强度的2倍：2T)的血管扩张反应可被毒碱胆碱能阻滞剂阿托品(阿托品)取消，而对高强度刺激(3T)的反应可被阿托品和烟碱胆碱能阻滞剂甲乙胺(Mecamylamine)取消。胆碱乙酰转移酶免疫细胞化学显示，当电极尖端位于含有胆碱能神经元的区域时，血流变化最大。这些结果表明，小鼠基底前脑神经元的胆碱能投射通过毒鼠碱和尼古丁机制的组合在同侧大脑皮质引起血管扩张，就像以前在大鼠和猫身上发现的那样。
We examined whether activity of the nucleus basalis of Meynert (NBM) regulates regional cerebral cortical blood flow (rCBF) in mice, using laser speckle and laser Doppler flowmetry. In anesthetized mice, unilateral focal stimulation, either electrical or chemical, of the NBM increased rCBF of the ipsilateral cerebral cortex in the frontal, parietal and occipital lobes, independent of changes in systemic blood pressure. Most of vasodilative responses to low intensity stimuli (2 times threshold intensity: 2T) were abolished by atropine (a muscarinic cholinergic blocker), whereas responses to higher intensity stimuli (3T) were abolished by atropine and mecamylamine (a nicotinic cholinergic blocker). Blood flow changes were largest when the tip of the electrode was located within the area containing cholinergic neurons shown by choline acetyl-transferase-immunocytochemistry. These results suggest that cholinergic projections from basal forebrain neurons in mice cause vasodilation in the ipsilateral cerebral cortex by a combination of muscarinic and nicotinic mechanisms, as previously found in rats and cats.