Enhancement of in vitro proliferation and bioactivity of human anterior cruciate ligament fibroblasts using an in situ tissue isolation method and basic fibroblast growth factor culture conditions A pilot analysis
Enhancement of in vitro proliferation and bioactivity of human anterior cruciate ligament fibroblasts using an in situ tissue isolation method and basic fibroblast growth factor culture conditions A pilot analysis
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利用原位组织分离方法和碱性成纤维细胞生长因子培养条件增强人前十字韧带成纤维细胞的体外增殖和生物活性初步分析
DOI：
10.1097/md.0000000000015907
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发表时间：
2019
期刊：
影响因子：
1.6
通讯作者：
Huang Wei
中科院分区：
文献类型：
--
作者：
Liu Ziming;Li Yuwan;Ren Youliang;Jin Ying;Yang Jibin;Wang Shengmin;Zhu Xizhong;Xiong Huazhang;Zou Gang;Liu Yi;Huang Wei
Background:Previous studies have reported poor proliferation and bioactivity of human anterior cruciate ligament fibroblasts (hACLFs) after injury. As hACLFs are one of the most significant and indispensable source of seed cells in constructing tissue-engineered ligament, enhancing hACLF proliferation would offer favorable cellular-biological ability and induce the extracellular matrix secretion of hACLFs after loading on multiple types of scaffolds. Enhancing the bioactivity of hACLFs would improve tissue repair and functional recovery after tissue-engineered ligament transplantation. This study compared cells prepared by collagenase digestion and the in situ culture of tissue pieces and investigated the effect of basic fibroblast growth factor (bFGF) on hACLFs.Methods:
