Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp nov from South-Western Siberian soda lakes
Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp nov from South-Western Siberian soda lakes
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DOI：
10.1007/s00792-008-0142-1
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发表时间：
2008-05-01
期刊：
影响因子：
2.9
通讯作者：
Muyzer, Gerard
中科院分区：
文献类型：
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作者：
Banciu, Horia L.;Sorokin, Dimitry Y.;Muyzer, Gerard
从俄罗斯阿拉泰西伯利亚的S-西伯利亚高盐碱湖中分离到一株能在近中性和极碱性pH条件下生长的化学自养型硫氧化细菌ALCO1。ALCO-1菌株是伽马变形杆菌中嗜盐细菌中的一个新的独立分支，到目前为止，它只含有中性嗜盐的SOB。根据其独特的表型特征和较远的系统发育关系，ALCO-1菌株被认为是一个新的属和种。11月SP.11月Alco-1能够在较宽的盐度范围内(全钠0.5-3.5M)生长，其最适生长条件为1M Na+，最适pH为7.2-10.2，pH(Opt)为8.5左右。中性(NaC L)培养的恒化器的最大生长速率(m u(Max))、呼吸活性和总细胞色素c含量明显低于碱性培养的恒化器。在中性pH和3M氯化钠溶液中产生的渗透调节物质(外生碱和甘氨酸甜菜碱)的比浓度大约是在pH为10的苏打溶液中的两倍。总之，菌株ALCO1代表了一种有趣的化学自养模式生物，用于比较研究细菌对高盐度和高pH的适应。
A chemolithoautotrophic sulfur-oxidizing bacterium (SOB) strain ALCO 1 capable of growing at both near-neutral and extremely alkaline pH was isolated from hypersaline soda lakes in S-W Siberia (Altai, Russia). Strain ALCO 1 represents a novel separate branch within the halothiobacilli in the Gammaproteobacteria, which, so far, contained only neutro-halophilic SOB. On the basis of its unique phenotypic properties and distant phylogeny, strain ALCO 1 is proposed as a new genus and species Thioalkalibacter halophilus gen. nov. sp. nov. ALCO 1 was able to grow within a broad range of salinity (0.5-3.5 M of total sodium) with an optimum at around 1 M Na+, and pH (7.2-10.2, pH(opt) at around 8.5). Na+ was required for sulfur-dependent respiration in ALCO 1. The neutral (NaCl)-grown chemostat culture had a much lower maximum growth rate (mu(max)), respiratory activity and total cytochrome c content than its alkaline-grown counterpart. The specific concentration of osmolytes (ectoine and glycine-betaine) produced at neutral pH and 3 M NaCl was roughly two times higher than at pH 10 in soda. Altogether, strain ALCO 1 represents an interesting chemolithoautotrophic model organism for comparative investigations of bacterial adaptations to high salinity and pH.