Monoxenic culture of the intraradical forms of Glomus sp. isolated from a tropical ecosystem:: a proposed methodology for germplasm collection
Monoxenic culture of the intraradical forms of Glomus sp. isolated from a tropical ecosystem:: a proposed methodology for germplasm collection
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DOI：
10.2307/3761216
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发表时间：
1998-07-01
期刊：
影响因子：
2.8
通讯作者：
Plenchette, C
中科院分区：
文献类型：
--
作者：
Declerck, S;Strullu, DG;Plenchette, C
从香蕉和甘蔗根际分离到5株丛枝菌根真菌，并将其与转基因胡萝卜根进行了联合培养。真菌作为菌根根片和单个孤立的囊泡的根内形式构成了良好的接种物来源，用于建立体外培养和连续培养的物种。获得了两个G. versiforme species，G. intraradices和G. fasciculatum，而G.大果。该共培养体系适合于AM真菌菌株的体外收集。
Five arbuscular mycorrhizal (AM) fungi, isolated from the rhizosphere of banana and sugar cane, were successfully cultured in vitro in association with genetically transformed roots of carrot. The intraradical forms of the fungi as mycorrhizal root pieces and single isolated vesicles constituted excellent sources of inoculum for the establishment of in vitro cultures and for the continuous culture of the species. Several thousand fungal propagules were obtained for the two G. versiforme species, G. intraradices and to a lesser extent for G. fasciculatum whereas few spores were obtained for G. macrocarpum. This coculture system appeared suitable for the establishment of in vitro collections of AM fungal strains.