ANALYSIS OF THE ESCHERICHIA-COLI GENOME - DNA-SEQUENCE OF THE REGION FROM 84.5 TO 86.5 MINUTES
ANALYSIS OF THE ESCHERICHIA-COLI GENOME - DNA-SEQUENCE OF THE REGION FROM 84.5 TO 86.5 MINUTES
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DOI：
10.1126/science.1379743
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发表时间：
1992-08-07
期刊：
影响因子：
56.9
通讯作者：
BLATTNER, FR
中科院分区：
文献类型：
--
作者：
DANIELS, DL;PLUNKETT, G;BLATTNER, FR
描述了大肠杆菌K-12基因组91.4kb的DNA序列，该序列跨越了遗传图谱上84.5分钟的rrnC和86.5分钟的rRNA之间的区域(物理图谱上85%到87%)。对该序列的分析确定了82个可能的编码区(开放阅读框)，覆盖了测序区间的84%。这些开放阅读框架的排列，加上计算机搜索发现的共同启动子序列和类似终止子的序列，使它们有可能被分配到拟议的转录单位。超过一半的开放阅读框与已知基因或功能相关，这是由于与其他序列的相似性所表明的。剩下的那些编码的蛋白质仍然是未知的。测序区还含有几个RNA基因，发现了两种重复序列元件。基因间隔区包括三个“灰洞”，大小在0.6到0.8千碱基之间，没有可识别的功能。
The DNA sequence of 91.4 kilobases of the Escherichia coli K-12 genome, spanning the region between rrnC at 84.5 minutes and rrnA at 86.5 minutes on the genetic map (85 to 87 percent on the physical map), is described. Analysis of this sequence identified 82 potential coding regions (open reading frames) covering 84 percent of the sequenced interval. The arrangement of these open reading frames, together with the consensus promoter sequences and terminator-like sequences found by computer searches, made it possible to assign them to proposed transcriptional units. More than half the open reading frames correlated with known genes or functions suggested by similarity to other sequences. Those remaining encode still unidentified proteins. The sequenced region also contains several RNA genes and two types of repeated sequence elements were found. Intergenic regions include three "gray holes," 0.6 to 0.8 kilobases, with no recognizable functions.