Effect of medium properties and additives on antibody stability and accumulation in suspended plant cell cultures
Effect of medium properties and additives on antibody stability and accumulation in suspended plant cell cultures
复制标题
培养基特性和添加剂对悬浮植物细胞培养物中抗体稳定性和积累的影响
DOI：
10.1111/j.1470-8744.2002.tb01186.x
复制
发表时间：
2002
影响因子：
2.8
通讯作者：
Pauline M. Doran
中科院分区：
文献类型：
--
作者：
Bonny M.‐Y. Tsoi;Pauline M. Doran
在转基因植物细胞培养物中研究了影响抗体积累和稳定性的因素。尽管IgG 1抗体稳定地保持在用于动物细胞培养的培养基中，但在加入无菌植物培养基中的抗体在1-2小时的时间内迅速损失。在不存在Mn的情况下，Gamborg B5培养基中的抗体稳定性得到改善。使用产生IgG 1抗体的烟草悬浮液来测试用于改善植物培养物中抗体积累的各种基于培养基的策略。尽管生长受到抑制，但在含有甘露醇的培养基中（渗透压摩尔浓度高达450 mOsm·（kg水）−1），生物质和培养基中的抗体水平增加。添加赤霉酸和氯化血红素的文化也是有益的，但效果不如那些在高渗条件下获得的。通过在不含Mn的B5培养基中培养植物细胞，发现抗体积累适度增加。
Factors affecting antibody accumulation and stability were investigated in transgenic plant cell cultures. Whereas IgG1 antibody was stably maintained in media used for animal cell culture, there was a rapid loss over a period of 1–2 h of antibody added to sterile plant culture media. Antibody stability in Gamborg's B5 medium was improved in the absence of Mn. Tobacco suspensions producing IgG1 antibody were used to test various medium‐based strategies for improving antibody accumulation in plant culture. Even though growth was suppressed, antibody levels in the biomass and medium were increased in media containing mannitol at osmolalities up to ≈450 mOsm·(kg of water)−1. Adding gibberellic acid and haemin to the cultures was also beneficial, but the effects were not as great as those obtained under hyperosmolar conditions. Moderate increases in antibody accumulation were found by culturing plant cells in B5 medium without Mn.
氯高铁血红素抑制高等植物和酵母中泛素依赖性蛋白水解作用。
影响因子：
2.9
作者：
Vierstra,RD;Sullivan,ML
通讯作者：
Sullivan,ML