Mouse but not zebrafish requires retinoic acid for control of neuromesodermal progenitors and body axis extension
Mouse but not zebrafish requires retinoic acid for control of neuromesodermal progenitors and body axis extension
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DOI：
10.1016/j.ydbio.2018.06.019
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发表时间：
2018-09-01
影响因子：
2.7
通讯作者：
Duester, Gregg
中科院分区：
文献类型：
--
作者：
Berenguer, Marie;Lancman, Joseph J.;Duester, Gregg
在小鼠中，视黄酸（RA）是由躯干中的神经中胚层祖细胞（NMPs）群体控制的体轴延伸的早期阶段所必需的，称为扩展-NMPs，而不是由尾部中的NMPs群体控制的体轴延伸的后期阶段所必需的，称为耗尽-NMPs。最近的观察表明，斑马鱼利用耗尽的NMPs，但不扩展的NMPs体轴延伸。在斑马鱼中，RA在体轴延伸中的作用不支持以前的研究缺乏RA合成的aldh 1a 2（raldh 2）突变体。在这里，通过用RA合成抑制剂处理斑马鱼胚胎，我们还发现体轴延伸和体节发生没有受到干扰，尽管观察到胸鳍和心脏水肿的损失与以前的研究一致。斑马鱼在体轴延伸不需要RA方面与小鼠不同的结论与斑马鱼缺乏早期扩展NMPs来生成躯干的结论一致。我们认为，RA控制的身体轴的延伸被添加到更高的脊椎动物在进化过程中扩展的NMPs。
In mouse, retinoic acid (RA) is required for the early phase of body axis extension controlled by a population of neuromesodermal progenitors (NMPs) in the trunk called expanding-NMPs, but not for the later phase of body axis extension controlled by a population of NMPs in the tail called depleting-NMPs. Recent observations suggest that zebrafish utilize depleting-NMPs but not expanding-NMPs for body axis extension. In zebrafish, a role for RA in body axis extension was not supported by previous studies on aldh1a2 (raldh2) mutants lacking RA synthesis. Here, by treating zebrafish embryos with an RA synthesis inhibitor, we also found that body axis extension and somitogenesis was not perturbed, although loss of pectoral fin and cardiac edema were observed consistent with previous studies. The conclusion that zebrafish diverges from mouse in not requiring RA for body axis extension is consistent with zebrafish lacking early expanding-NMPs to generate the trunk. We suggest that RA control of body axis extension was added to higher vertebrates during evolution of expanding-NMPs.