Increased serum cutaneous T cell-attracting chemokine (CCL27) levels in patients with atopic dermatitis and psoriasis vulgaris
Increased serum cutaneous T cell-attracting chemokine (CCL27) levels in patients with atopic dermatitis and psoriasis vulgaris
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DOI：
10.1067/mai.2003.114
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发表时间：
2003-03-01
影响因子：
14.2
通讯作者：
Tamaki, K
中科院分区：
文献类型：
--
作者：
Kakinuma, T;Saeki, H;Tamaki, K
特应性皮炎（AD）和寻常性牛皮癣（PsV）都是一种慢性和复发性炎症性皮肤病，与各种免疫异常有关。皮肤T细胞吸引趋化因子（CTACK; CCL27）是CC趋化因子家族的成员，也是CC趋化因子受体10的功能配体。它在皮肤中选择性表达，并吸引表达CC趋化因子受体10的皮肤归巢记忆T细胞。表皮角质形成细胞是CTACK的主要来源，提示与多种炎症性皮肤病有关。目的：本研究旨在明确AD和PsV患者血清中是否检测到角质形成细胞产生的CTACK，并探讨AD和PsV患者血清CTACK水平与疾病活动度的相关性。方法：测定50例AD患者、30例PsV患者和22例健康对照者血清CTACK水平。我们还将50例AD患者分为3组（即轻度、中度和重度疾病），并在3个类别中进行比较。此外，我们将AD和PsV患者的血清CTACK水平与临床或实验室数据进行了比较。在AD和PsV患者的病变皮肤上进行CTACK和ifn诱导的10 kd蛋白（IP-10; CXCL10）的免疫组化染色。结果：AD和PsV患者血清CTACK水平显著高于健康对照组。AD患者血清CTACK水平与特应性皮炎（SCORAD）评分、血清可溶性IL-2受体水平、血清可溶性e-选择素水平、血清胸腺和活化调节趋化因子水平、血清巨噬细胞来源趋化因子水平显著相关。PsV患者血清CTACK水平与血清IP-10水平显著相关，但与银屑病面积和严重程度指数评分无显著相关性。免疫组化染色显示CTACK在AD和PsV患者的病变角质形成细胞中强烈表达，而IP-10在PsV患者的病变角质形成细胞中强烈表达，在AD患者中局部表达。结论：CTACK可能是AD和PsV发病的重要趋化因子之一。
Both atopic dermatitis (AD) and psoriasis vulgaris (PsV) are characterized as chronic and relapsing inflammatory skin diseases associated with various immunologic abnormalities. Cutaneous T cell-attracting chemokine (CTACK; CCL27) is a member of the CC chemokine family and a functional ligand for CC chemokine receptor 10. It is selectively expressed in skin and attracts CC chemokine receptor 10-expressing skin-homing memory T cells. The epidermal keratinocyte is a main source of CTACK, suggesting the involvement of various inflammatory skin diseases.Objective: The purpose of this investigation was to clarify whether CTACK produced by keratinocytes is detected in the sera of patients with AD and PsV and to examine the correlation between the serum CTACK levels and disease activity of patients with AD and PsV.Methods: We measured the serum CTACK levels in 50 patients with AD, 30 patients with PsV, and 22 healthy control subjects. We also divided 50 patients with AD into 3 groups (ie, those with mild, moderate, and severe disease) and compared them among 3 categories. Moreover, we compared the serum CTACK levels of patients with AD and PsV with clinical or laboratory data. Immunohistochemical staining of CTACK and IFN-induced protein of 10 kd (IP-10; CXCL10) was performed on the lesional skin of patients with AD and PsV.Results: The serum CTACK levels in,patients with AD and PsV were significantly higher than those in healthy control subjects. The serum CTACK levels in patients with AD significantly correlated with scoring atopic dermatitis (SCORAD) scores, serum soluble IL-2 receptor levels, serum soluble E-selectin levels, serum thymus and activation-regulated chemokine levels, and serum macrophage-derived chemokine levels. Serum CTACK levels in patients with PsV significantly correlated with the serum IP-10 levels but not with the Psoriasis Area and Severity Index score. Immunohistochemical staining showed CTACK was strongly expressed in lesional keratinocytes of patients with AD and PsV, whereas IP-10 was strongly expressed in lesional keratinocytes of patients with PsV and focally in those with AD.Conclusion: These results suggest that CTACK might be one of the important chemokines for the pathogenesis of AD and PsV.