BnaC7.ROT3, the causal gene of cqSL-C7, mediates silique length by affecting cell elongation in Brassica napus
BnaC7.ROT3, the causal gene of cqSL-C7, mediates silique length by affecting cell elongation in Brassica napus
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DOI：
10.1093/jxb/erab407
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发表时间：
2021-09-06
影响因子：
6.9
通讯作者：
Hong, Dengfeng
中科院分区：
文献类型：
--
作者：
Zhou, Xianming;Zhang, Haiyan;Hong, Dengfeng
角果是油菜（Brassica napus）种子发育后期的主要碳水化合物能量来源。因此，角果长度受到育种者的极大关注。我们先前在B中检测到一个控制角果长度的新的数量性状位点cqSL-C7。油菜。在此，我们进一步验证了cqSL-C7位点，并通过图位克隆分离了其致病基因（BnaC7.ROT3）。在“中双11”（长角果亲本）中，BnaC7.ROT3编码潜在的细胞色素P450单加氧酶CYP 90 C1，而在“G120”（短角果亲本）中，BnaC7.ROT3第五外显子的单核苷酸缺失导致功能丧失的截短蛋白。亚细胞定位和表达模式分析表明，BnaC7.ROT3是一个膜定位蛋白，主要在叶，花和角果中表达。细胞学观察表明，BnaC 7细胞长角果状壁。在‘G120’背景下，ROT 3转化的阳性植株比转基因阴性植株长，表明BnaC7.ROT3影响细胞伸长。单倍型分析表明BnaC 7. ROT 3在B中是有利的。napus胚粒及其同源物也可能参与角果长度的调节。本研究为揭示油菜角果长度变异的调控机制提供了新的思路，也为油菜角果长度的改良提供了宝贵的遗传资源。
Siliques are a major carbohydrate source of energy for later seed development in rapeseed (Brassica napus). Thus, silique length has received great attention from breeders. We previously detected a novel quantitative trait locus cqSL-C7 that controls silique length in B. napus. Here, we further validated the cqSL-C7 locus and isolated its causal gene (BnaC7.ROT3) by map-based cloning. In `Zhongshuang11' (parent line with long siliques), BnaC7.ROT3 encodes the potential cytochrome P450 monooxygenase CYP90C1, whereas in `G120' (parent line with short siliques), a single nucleotide deletion in the fifth exon of BnaC7.ROT3 results in a loss-of-function truncated protein. Sub-cellular localization and expression pattern analysis revealed that BnaC7.ROT3 is a membrane-localized protein mainly expressed in leaves, flowers and siliques. Cytological observations showed that the cells in silique walls of BnaC7. ROT3-transformed positive plants were longer than those of transgene-negative plants in the background of `G120', suggesting that BnaC7.ROT3 affects cell elongation. Haplotype analysis demonstrated that most alleles of BnaC7. ROT3 are favorable in B. napus germplasms, and its homologs may also be involved in silique length regulation. Our findings provide novel insights into the regulatory mechanisms of natural silique length variations and valuable genetic resources for the improvement of silique length in rapeseed.