An aqueous-organic two-phase bioprocess for efficient production of the natural aroma chemicals 2-phenylethanol and 2-phenylethylacetate with yeast
An aqueous-organic two-phase bioprocess for efficient production of the natural aroma chemicals 2-phenylethanol and 2-phenylethylacetate with yeast
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DOI：
10.1007/s00253-005-0281-6
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发表时间：
2006-07-01
影响因子：
5
通讯作者：
Schrader, J.
中科院分区：
文献类型：
--
作者：
Etschmann, M. M. W.;Schrader, J.
天然芳香化学品2-苯基乙醇（2-PE）和2-苯基乙基乙酸酯（2-PEAc）具有高度的工业相关性，并且可以在基于酵母的过程中由L-苯丙氨酸生产，并形成生长相关的产物。由于产物抑制，原位产物去除是强制性的，以获得经济上令人感兴趣的浓度。使用聚丙二醇1200作为原位提取剂和饱和浓度的前体的分批补料方法导致迄今为止生物过程中报道的最高2-PE生产率。用马克斯克鲁维酵母CBS 600，在有机相中获得26.5g/l 2-PE和6.1g/l 2-PEAc，分别对应于0.33和0.08g/l h的时空产率。
The natural aroma chemicals 2-phenylethanol (2-PE) and 2-phenylethylacetate (2-PEAc) are of high industrial relevance and can be produced from L-phenylalanine in a yeast-based process with growth-associated product formation. Due to product inhibition, in situ product removal is mandatory to obtain economically interesting concentrations. A fed-batch approach using polypropylene glycol 1200 as in situ extractant and the precursor in a saturated concentration led to the highest 2-PE productivity reported for a bioprocess so far. With Kluyveromyces marxianus CBS 600, 26.5 g/l 2-PE and 6.1 g/l 2-PEAc in the organic phase were obtained, corresponding to space-time yields of 0.33 and 0.08 g/l h, respectively.