Molecular evidence for heterogeneity of the multiple-drug-resistant Mycobacterium tuberculosis population in Scotland (1990 to 1997)
Molecular evidence for heterogeneity of the multiple-drug-resistant Mycobacterium tuberculosis population in Scotland (1990 to 1997)
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DOI：
10.1128/jcm.37.4.998-1003.1999
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发表时间：
1999-04-01
影响因子：
9.4
通讯作者：
Forbes, KJ
中科院分区：
文献类型：
--
作者：
Fang, Z;Doig, C;Forbes, KJ
多重耐药结核分枝杆菌（MDR-MTB）已在医院或卫生保健机构和人类免疫缺陷病毒感染人群中得到充分研究。然而，目前对MDR-MTB在社区中的流行特征还没有得到很好的研究，了解其在社区中的流行和传播情况，将有助于评估MDR-MTB在社区中的流行情况，并优化其发展和传播的控制和预防策略。在这项研究中，MDR-MTB分离株从1990年和1997年之间收集的特点，沿着与非耐药菌株。结果表明，它们的基因是多样的，这表明它们彼此无关，可能是独立进化的。rpoB、katG和ahpC的几个新等位基因被鉴定：rpoB密码子525（ACC->AAC; Thr 525 Asn）; katG密码子128（CGG->CAG; Arg 128 Gln）和密码子291（GCT->CCT; Ala 291 Pro）;以及ahpC在密码子G的同义替换（ATT->ATC）。其中一株来自亚洲患者的MDR-MTB分离株具有与MDR-MTR W株非常相似的IS 6110限制性片段长度多态性模式，并具有相同的耐药相关等位基因，但与W株没有任何流行病学联系。此外，还发现了M.在我们收集的715个临床分离株中鉴定了结核分离株;该簇中的分离株具有与W株非常相似的遗传背景，其中一个已经发展出多重耐药性。结核病，对控制生物体和预防其传播具有意义。
Multiple-drug-resistant Mycobacterium tuberculosis (MDR-MTB) has been well studied in hospitals or health care institutions and in human immunodeficiency virus-infected populations. However, the characteristics of MDR-MTB in the community have not been well investigated, An understanding of its prevalence and circulation within the community will help to estimate the problem and optimize the strategies for control and prevention of its development and transmission. In this study, MDR-MTB isolates from Scotland collected between 1990 and 1997 were characterized, along with non-drug-resistant isolates. The results showed that they were genetically diverse, suggesting they were unrelated to each other and had probably evolved independently. Several new alleles of rpoB, katG, and ahpC were identified: rpoB codon 525 (ACC-->AAC; Thr525Asn); katG codon 128 (CGG-->CAG; Arg128Gln) and codon 291 (GCT-->CCT; Ala291Pro); and the ahpC synonymous substitution at codon G (ATT-->ATC). One of the MDR-MTB isolates from an Asian patient had an IS6110 restriction fragment length polymorphism pattern very similar to that of the MDR-MTR W strain and had the same drug resistance-related alleles but did not have any epidemiological connection with the W strains. Additionally, a cluster of M. tuberculosis isolates was identified in our collection of 715 clinical isolates; the isolates in this cluster had genetic backgrounds very similar to those of the W strains, one of which had already developed multiple drug resistances, The diverse population of MDR-MTB in Scotland, along with a low incidence of drug-resistant M. tuberculosis, has implications fur the control of the organism and prevention of its spread.