Mechanical force augments the anti-osteoclastogenic potential of human gingival fibroblasts in vitro.
Mechanical force augments the anti-osteoclastogenic potential of human gingival fibroblasts in vitro.
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DOI：
10.1111/j.1600-0765.2008.01121.x
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发表时间：
2009-06
影响因子：
3.5
通讯作者：
S. Kook;Young-Ok Son;Y. Choe;J.H. Kim;Young-Mi Jeon;J. Heo;J-G Kim;J. Lee
中科院分区：
文献类型：
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作者：
S. Kook;Young-Ok Son;Y. Choe;J.H. Kim;Young-Mi Jeon;J. Heo;J-G Kim;J. Lee
背景与目的人牙龈成纤维细胞对机械力的细胞反应被认为主要是抗骨细胞的，因为它们产生相对高水平的骨保护素。然而，关于压缩力对这些细胞产生骨保护素和骨细胞分化的影响的信息很少。在这项研究中，我们研究了机械力如何影响人类牙龈成纤维细胞的性质，以产生护骨素和抑制破骨细胞生成。材料与方法将人牙龈成纤维细胞以约50 g/cm（2）的量级离心90 min，暴露于机械力。在施加力后的不同时间点测量骨保护素、核因子-κ B受体激活剂配体（RANKL）、白细胞介素-1 β和肿瘤坏死因子-α的水平。还使用人牙龈成纤维细胞和骨髓细胞的共培养系统确定离心力对破骨细胞样细胞形成的影响。结果离心力刺激细胞表达骨保护素、RANKL、白细胞介素1 β和肿瘤坏死因子α，并在蛋白水平产生较高的骨保护素/RANKL比值。白细胞介素-1 β和肿瘤坏死因子-α均加速了力诱导的骨保护素的产生，而加入抗（白细胞介素-1 β）免疫球蛋白IG同种型; IgG（兔多克隆）可显著抑制骨保护素的产生。然而，添加抗（肿瘤坏死因子-α）免疫球蛋白IG同种型; IgG 1（小鼠单克隆）无影响。离心力对破骨细胞的形成也有抑制作用。结论离心力可促进牙龈成纤维细胞产生骨保护素，从而刺激牙龈成纤维细胞抑制破骨细胞生成。总的来说，人类牙龈成纤维细胞可能具有天然的防御机制，以抑制由机械应力引起的骨吸收。
BACKGROUND AND OBJECTIVE The cellular response of human gingival fibroblasts to a mechanical force is considered to be primarily anti-osteoclastic because they produce relatively high levels of osteoprotegerin. However, there is little information available on the effects of compression force on the production of osteoprotegerin and osteoclastic differentiation by these cells. In this study, we examined how mechanical force affects the nature of human gingival fibroblasts to produce osteoprotegerin and inhibit osteoclastogenesis. MATERIAL AND METHODS Human gingival fibroblasts were exposed to mechanical force by centrifugation for 90 min at a magnitude of approximately 50 g/cm(2). The levels of osteoprotegerin, receptor activator of nuclear factor-kappaB ligand (RANKL), interleukin-1beta and tumor necrosis factor-alpha were measured at various time-points after applying the force. The effect of the centrifugal force on the formation of osteoclast-like cells was also determined using a co-culture system of human gingival fibroblasts and bone marrow cells. RESULTS Centrifugal force stimulated the expression of osteoprotegerin, RANKL, interleukin-1beta and tumor necrosis factor-alpha by the cells, and produced a relatively high osteoprotegerin to RANKL ratio at the protein level. Both interleukin-1beta and tumor necrosis factor-alpha accelerated the force-induced production of osteoprotegerin, which was inhibited significantly by the addition of anti-(interleukin-1beta) immunoglobulin Ig isotype; IgG (rabbit polyclonal). However, the addition of anti-(tumor necrosis factor-alpha) immunoglobulin Ig isotype; IgG1 (mouse monoclonal) had no effect. Centrifugal force also had an inhibitory effect on osteoclast formation. CONCLUSION Application of centrifugal force to human gingival fibroblasts accelerates osteoprotegerin production by these cells, which stimulates the potential of human gingival fibroblasts to suppress osteoclastogenesis. Overall, human gingival fibroblasts might have natural defensive mechanisms to inhibit bone resorption induced by a mechanical stress.