Atmospheric scanning electron microscope system with an open sample chamber: Configuration and applications
Atmospheric scanning electron microscope system with an open sample chamber: Configuration and applications
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DOI：
10.1016/j.ultramic.2014.06.001
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发表时间：
2014-12-01
期刊：
影响因子：
2.2
通讯作者：
Sato, Chikara
中科院分区：
文献类型：
--
作者：
Nishiyama, Hidetoshi;Koizumi, Mitsuru;Sato, Chikara
本文介绍了一种带有开放式样品室和光学显微镜的大气扫描电子显微镜（ASEM）及其最新进展。在该ClairScope系统中，开放式样品盘的底部密封在倒置的SEM柱的顶部，允许从上方通过OM和从下方通过SEM观察液体浸没的样品。两个显微镜的光轴对齐，确保相同的样品区域成像，以实现溶液中的准同时相关显微镜。例如，直接展示了ZnO颗粒的阴极射线发光。改进的系统具有（i）完全机动化的样品台，（ii）在意外窗户破裂的情况下的柱保护系统，以及（iii）由图形用户界面控制的OM/SEM操作系统。开放式样品室允许在样品观察期间进行试剂的外部给药。我们监测了添加的氯化钠对液体中二氧化硅颗粒随机运动的影响。此外，使用荧光作为转染标记，检查了小干扰RNA介导的内源性Varp敲低对黑素细胞中Tyrpl运输的影响。温度调节的钛ASEM盘允许动态观察胶体银纳米颗粒，因为它们被加热到240 ℃-循环和烧结。(C)2014 Elsevier BY. All rights reserved.
An atmospheric scanning electron microscope (ASEM) with an open sample chamber and optical microscope (OM) is described and recent developments are reported. In this ClairScope system, the base of the open sample dish is sealed to the top of the inverted SEM column, allowing the liquid-immersed sample to be observed by OM from above and by SEM from below. The optical axes of the two microscopes are aligned, ensuring that the same sample areas are imaged to realize quasi-simultaneous correlative microscopy in solution. For example, the cathodoluminescence of ZnO particles was directly demonstrated. The improved system has (i) a fully motorized sample stage, (ii) a column protection system in the case of accidental window breakage, and (iii) an OM/SEM operation system controlled by a graphical user interface. The open sample chamber allows the external administration of reagents during sample observation. We monitored the influence of added NaCI on the random motion of silica particles in liquid. Further, using fluorescence as a transfection marker, the effect of small interfering RNA mediated knockdown of endogenous Varp on Tyrpl trafficking in melanocytes was examined. A temperature regulated titanium ASEM dish allowed the dynamic observation of colloidal silver nanoparticles as they were heated to 240 C-circle and sintered. (C) 2014 Elsevier BY. All rights reserved.