A QCM immunosensor for Salmonella detection with simultaneous measurements of resonant frequency and motional resistance
A QCM immunosensor for Salmonella detection with simultaneous measurements of resonant frequency and motional resistance
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DOI：
10.1016/j.bios.2005.01.021
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发表时间：
2005-12-15
影响因子：
12.6
通讯作者：
Li, YB
中科院分区：
文献类型：
--
作者：
Su, XL;Li, YB
介绍了一种同时测量共振频率和动态电阻的石英晶体微天平（QCM）免疫传感器。免疫传感器的制备使用蛋白A的抗体固定。高频阻抗分析表明，QCM的谐振频率和动态电阻（Δ F和Δ R）的变化是显着的，而静态电容，动态电容，和动态电感的变化是不显着的。在直接检测S.鸡肉样品中的鼠伤寒沙门氏菌、Δ F和Δ R分别在10（5）~ 10（8）和10（6）~ 10（8）cells/ml范围内与细胞浓度成正比。使用抗沙门氏菌磁珠作为样品预处理的分离器/浓缩器以及信号放大的标记物，基于Δ R测量，检测限降低至10（2）个细胞/ml;然而，Δ F与细胞浓度无关。未观察到E. coli K12或样品基质。0 2005 Elsevier B. V.保留所有战斗。
A quartz crystal microbalance (QCM) immunosensor was described for the detection of Salmonella Typhimurium with simultaneous measurements of the resonant frequency and motional resistance. The immunosensor was fabricated using protein A for the antibody immobilization. High-frequency impedance analysis indicated that the changes in resonant frequency and motional resistance (Delta F and Delta R) of the QCM were significant while the changes in static capacitance, motional capacitance, and motional inductance were insignificant. In the direct detection of S. Typhimurium in chicken meat sample, Delta F and Delta R were proportional to the cell concentration in the range of 10(5)-10(8) and 10(6)-10(8) cells/ml, respectively. Using anti-Salmonella-magnetic beads as a separator/concentrator for sample pretreatment as well as a marker for signal amplification, the detection limit was lowered to 10(2) cells/ml based on the Delta R measurement; however, Delta F was not related to the cell concentration. No interference was observed from E. coli K12 or the sample matrix. 0 2005 Elsevier B.V. All fights reserved.