Proliferating cell nuclear antigen in blast crisis cells of patients with chronic myeloid leukemia.
Proliferating cell nuclear antigen in blast crisis cells of patients with chronic myeloid leukemia.
复制标题
慢性粒细胞白血病患者急变细胞中的增殖细胞核抗原。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
E. Tan
中科院分区：
文献类型：
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作者：
Y. Takasaki;W. Robinson;E. Tan
一种与细胞增殖相关的核抗原[增殖细胞核抗原（PCNA）]和原始细胞转化可被某些系统性红斑狼疮患者血清中的自身抗体识别;这些自身抗体是沉淀抗体并且也在免疫荧光中反应，一种用于确定慢性粒细胞白血病（CML）患者白细胞中是否表达PCNA的技术在疾病的某些阶段。在任何时候，抗PCNA抗体染色的细胞百分比与外周血白细胞中原始细胞百分比之间都存在很强的相关性（r）= 0.865，P <0.001。但在急变期，某些形态学上类似于中粒细胞、晚幼粒细胞和部分核分节的细胞被抗PCNA血清染色。这种染色，保持核的位置，是不太强烈的比在母细胞，这表明在这些非母细胞抗原的低密度。在从缓解期患者获得的髓细胞或晚幼粒细胞中未观察到这种现象。这些初步的研究表明，抗PCNA可作为一种试剂，以检测CML危象中的原始细胞，也有能力检测与原始细胞危象相关的其他细胞中的PCNA。
A nuclear antigen associated with cell proliferation [proliferating cell nuclear antigen (PCNA)] and blast transformation is recognized by autoantibodies in the sera of some patients with systemic lupus erythematosus; these autoantibodies are precipitating antibodies and also react in immunofluorescence, a technique that was used to determine if PCNA might be expressed in leukocytes of patients with chronic myeloid leukemia (CML) during certain phases of their disease. At all times, a strong relationship was seen between the percentage of cells stained by anti-PCNA antibody and the percentage of blast cells in peripheral blood leukocytes (r) = 0.865, P less than .001. However, during blast crisis, certain cells that morphologically looked like myelocytes, metamyelocytes, and some cells with segmented nuclei stained with anti-PCNA serum. This staining, which remained nuclear in location, was less intense than in blast cells, suggesting low density of the antigen in these nonblast cells. This phenomenon was not observed in myelocytes or metamyelocytes obtained from patients in remission. These initial studies demonstrated that anti-PCNA can be used as a reagent to detect blast cells in CML crisis and also has the capability to detect PCNA in other cells associated with blast crisis.