Detection of respiratory viruses and subtype identification of influenza a viruses by GreeneChipResp oligonucleotide microarray
Detection of respiratory viruses and subtype identification of influenza a viruses by GreeneChipResp oligonucleotide microarray
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DOI：
10.1128/jcm.00737-07
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发表时间：
2007-08-01
影响因子：
9.4
通讯作者：
Lipkin, W. Ian
中科院分区：
文献类型：
--
作者：
Quan, Phenix-Lan;Palacios, Gustavo;Lipkin, W. Ian
急性呼吸道感染是世界范围内发病率、死亡率和经济负担的重要原因。一个准确的，早期的鉴别诊断可能会改变个人的临床管理，以及促进识别的爆发，对公共卫生的影响。在这里，我们报告的建立和验证一个全面和敏感的微阵列系统检测呼吸道病毒和流感病毒亚型的临床材料。一组流感病毒富集引物的实施通过血凝素1至16和神经氨酸酶1至9的差异识别促进了甲型流感病毒的亚型分型。21种不同的呼吸道病毒物种得到了准确的鉴定，包括最近发现的一种新的鼻病毒遗传分支。
Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus.