In vitro transformation of mesenchymal cells derived from embryonic muscle into cartilage in response to extracellular matrix components of bone.
In vitro transformation of mesenchymal cells derived from embryonic muscle into cartilage in response to extracellular matrix components of bone.
复制标题
来自胚胎肌肉的间充质细胞响应于骨的细胞外基质成分而在体外转化为软骨。
DOI：
10.1073/pnas.81.11.3419
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
Reddi,AH
中科院分区：
文献类型：
--
作者：
Sampath,TK;Nathanson,MA;Reddi,AH
大鼠骨干脱钙骨基质皮下植入诱导软骨和骨形成。当骨骼肌碎料在脱钙骨半圆柱上体外培养时，间充质细胞转化为软骨细胞。在本研究中，骨细胞外基质成分在体外触发软骨分化的潜力进行了研究。用6 M胍X HCl提取骨半圆柱导致体外软骨形成和体内软骨内骨形成的情况下。然后用胍提取物和从骨基质中提取的部分纯化组分重建生物学上无活性的骨半圆柱体并进行生物测定。重建完全恢复了体外诱导软骨形成和体内软骨内骨分化的能力。重建的整个胍提取物上的微孔过滤器涂有凝胶的肌腱胶原蛋白（I型）和随后的培养与切碎的骨骼肌也导致在体外软骨诱导。这些观察结果表明，骨的细胞外基质是一个知识库的因素，管理当地的软骨和骨分化。
Subcutaneous implantation of demineralized diaphyseal bone matrix into rats induces cartilage and bone formation in vivo. When minced skeletal muscle is cultured on hemicylinders of demineralized bone in vitro, mesenchymal cells are transformed into chondrocytes. In the present investigation, the potential of extracellular matrix components of bone to trigger cartilage differentiation in vitro was examined. Extraction of bone hemicylinders with 6 M guanidine X HCl resulted in the absence of chondrogenesis in vitro and endochondral bone formation in vivo. Biologically inactive hemicylinders of bone were then reconstituted with the guanidine extract and also with partially purified components extracted from bone matrix and bioassayed. Reconstitution completely restored the ability to elicit chondrogenesis in vitro and endochondral bone differentiation in vivo. Reconstitution of the whole guanidine extract on Millipore filters coated with gels of tendon collagen (type I) and subsequent culture with minced skeletal muscle also resulted in cartilage induction in vitro. These observations show that the extracellular matrix of bone is a repository of factors that govern local cartilage and bone differentiation.