CD68+macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining
CD68+macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining
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DOI：
10.1002/jbm.b.34639
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发表时间：
2020-05-30
影响因子：
3.4
通讯作者：
Davies, Luke
中科院分区：
文献类型：
--
作者：
Klinge, Uwe;Dievernich, Axel;Davies, Luke
植入物，如网状物，用于加强组织，实现持久炎症的形成与周围的纤维化反应。在炎性浸润中已经发现了几种不同的细胞类型，但CD68+巨噬细胞被认为是最重要的。为了研究浸润物中不同类型细胞之间的协同作用，我们用CD68作为恒定标记，用多种其他抗体作为第二标记，对人外植网进行了双荧光染色。第二标记包括淋巴细胞（CD3、CD4、CD8、CD16、CD56、FoxP3和CD11b）、干细胞（CD34）、白细胞（CD45、CD15）、巨噬细胞（CD86、CD105、CD163和CD206）；EC基质沉积（胶原- i、胶原- iii、MMP2和MMP8）；Ki67作为增殖标志物；酪氨酸蛋白激酶受体AXL。本研究表明，在炎症浸润中，CD68+细胞具有丰富的共表达多种其他标记物的能力，包括淋巴细胞的标记物，占所研究细胞的5%至83%。这种共染现象并不局限于一种特定的聚合物，在聚丙烯纤维和聚偏氟乙烯纤维中也可以观察到，尽管共表达率有所不同。这些细胞的持续变异性，没有向分化的成熟细胞类型的功能减少，可能有利于在网状界面缺乏愈合。
Implants like meshes for the reinforcement of tissues implement the formation of a persistent inflammation with an ambient fibrotic reaction. In the inflammatory infiltrate several distinct cell types have been identified, but CD68+ macrophages are supposed to be most important. To investigate the collaboration among the various cell types within the infiltrate we performed at explanted meshes from humans double fluorescence staining with CD68 as a constant marker and a variety of other antibodies as the second marker. The list of second markers includes lymphocytes (CD3, CD4, CD8, CD16, CD56, FoxP3, and CD11b) stem cells (CD34), leucocytes (CD45, CD15), macrophages (CD86, CD105, CD163, and CD206); deposition of EC matrix (collagen-I, collagen-III, MMP2, and MMP8); Ki67 as a marker for proliferation; and the tyrosine-protein kinase receptor AXL. The present study demonstrates within the inflammatory infiltrate the abundant capability of CD68+ cells to co-express a huge variety of other markers, including those of lymphocytes, varying between 5 and 83% of investigated cells. The observation of co-staining was not restricted to a specific polymer but was seen with polypropylene fibers as well as with fibers made of polyvinylidene fluoride, although with differences in co-expression rates. The persisting variability of these cells without the functional reduction toward differentiated mature cell types may favor the lack of healing at the interface of meshes.