Changes in the microbial community in Japan Trench sediment from a depth of 6292 m during cultivation without decompression.
Changes in the microbial community in Japan Trench sediment from a depth of 6292 m during cultivation without decompression.
复制标题
日本6292米深度的海沟沉积物在不减压培养过程中微生物群落的变化。
作者：
Miki Yanagibayashi;Y. Nogi;Lina Li;C. Kato
使用保压沉积物取样器从日本海沟6 292米深处采集了深海沉积物样品。沉积物样品中的微生物培养在海洋肉汤2216在环境压力（65 MPa）不减压，并在大气压力（0.1 MPa）作为对照实验。从原始沉积物样品和混合培养物中提取DNA，PCR扩增16S核糖体RNA基因（rDNA），并测定其核苷酸序列。基于16S rDNA序列的系统发育分析结果表明，原始沉积物样品中微生物多样性在细菌域中的类型分布较广。此外，在65 MPa下培养的混合培养物中，在培养开始时属于希瓦氏菌嗜压分支和Moritella属的细菌菌株一起存在，并且Moritella菌株在培养期结束时成为优势菌株。最后，在大气压下孵育的混合培养物中，属于假单胞菌属的菌株在任何时候都占优势。从培养物中提取的脂肪酸分析支持系统发育的结果。
A sample of deep-sea sediment was obtained from the Japan Trench at a depth of 6292 m using a pressure-retaining sediment sampler. Microorganisms in the sediment sample were cultivated in marine broth 2216 at ambient pressure (65 MPa) without decompression, and at atmospheric pressure (0.1 MPa) as a control experiment. 16S ribosomal RNA genes (rDNA) were amplified by PCR from DNA extracted from the original sediment sample and the mixed cultures, and the nucleotide sequences were determined. The results of phylogenetic analysis based on 16S rDNA sequences indicated that microbial diversity in the original sediment samples showed a wide distribution of types in the domain Bacteria. Furthermore, in the mixed cultures incubated at 65 MPa without decompression, bacterial strains belonging to the Shewanella barophiles branch and the genus Moritella existed together at the beginning of cultivation, and Moritella strains became dominant towards the end of the cultivation period. Finally, in the mixed cultures incubated at atmospheric pressure, strains belonging to the genus Pseudomonas were dominant at all times. Analysis of fatty acids extracted from the cultures supported the phylogenetic results.