Heat shock factor 1 upregulates transcription of Epstein-Barr Virus nuclear antigen 1 by binding to a heat shock element within the BamHI-Q promoter.
Heat shock factor 1 upregulates transcription of Epstein-Barr Virus nuclear antigen 1 by binding to a heat shock element within the BamHI-Q promoter.
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DOI：
10.1016/j.virol.2011.10.001
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发表时间：
2011-12
期刊：
影响因子：
3.7
通讯作者：
Feng-wei Wang;Xian-rui Wu;Wen-Ju Liu;Y. Liao;Sheng Lin;Y. Zong;M. Zeng;Y. Zeng;S. Mai;D. Xie
中科院分区：
文献类型：
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作者：
Feng-wei Wang;Xian-rui Wu;Wen-Ju Liu;Y. Liao;Sheng Lin;Y. Zong;M. Zeng;Y. Zeng;S. Mai;D. Xie
EB病毒（EBV）核抗原1（EBNA 1）是维持附加体和建立潜伏期所必需的。在这项研究中，我们观察到，热处理有效地诱导EBNA 1在EBV转化的B 95 -8和人LCL细胞系的转录。尽管Cp被认为是EBNA 1转录本在淋巴母细胞系中表达的唯一启动子，但RT-PCR结果表明，热处理诱导的EBNA 1转录本来自于Qp启动的转录本。利用生物信息学，在Qp的−17/+4寡核苷酸内发现了一个高亲和力和功能性的热休克因子1（HSF 1）结合元件，并通过电泳迁移率变动分析和染色质免疫沉淀分析确定。此外，热休克和外源性HSF 1表达诱导Qp活性的报告分析。此外，RNA干扰介导的HSF 1基因沉默减弱了B 95 -8细胞中热诱导的EBNA 1表达。这些结果为EBNA 1是转录因子HSF 1的新靶点提供了证据。
Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) is essential for maintenance of the episome and establishment of latency. In this study, we observed that heat treatment effectively induced EBNA1 transcription in EBV-transformed B95-8 and human LCL cell lines. Although Cp is considered as the sole promoter used for the expression of EBNA1 transcripts in the lymphoblastoid cell lines, the RT-PCR results showed that the EBNA1 transcripts induced by heat treatment arise from Qp-initiated transcripts. Using bioinformatics, a high affinity and functional heat shock factor 1 (HSF1)-binding element within the −17/+4 oligonucleotide of the Qp was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Moreover, heat shock and exogenous HSF1 expression induced Qp activity in reporter assays. Further, RNA interference-mediated HSF1 gene silencing attenuated heat-induced EBNA1 expression in B95-8 cells. These results provide evidence that EBNA1 is a new target for the transcription factor HSF1.