AtPER1 enhances primary seed dormancy and reduces seed germination by suppressing the ABA catabolism and GA biosynthesis in Arabidopsis seeds
AtPER1 enhances primary seed dormancy and reduces seed germination by suppressing the ABA catabolism and GA biosynthesis in Arabidopsis seeds
复制标题
AtPER1 通过抑制拟南芥种子中 ABA 分解代谢和 GA 生物合成来增强初级种子休眠并减少种子萌发
DOI：
10.1111/tpj.14542
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发表时间：
2019-10-22
期刊：
影响因子：
7.2
通讯作者：
Huang, Shangzhi
中科院分区：
文献类型：
--
作者：
Chen, Huhui;Ruan, Jiuxiao;Huang, Shangzhi
种子对于种质资源和植物生物多样性的保存至关重要。种子休眠是许多种子植物物种的一种适应性性状，使植物能够在逆境条件下生存。种子休眠主要受脱落酸（阿坝）和赤霉素（GA）的控制，可分为初级休眠和次级休眠。种子休眠主要由母体阿坝诱导。在这里，我们发现，AtPER 1，种子特异性过氧化物酶，参与提高初级种子休眠。两个atper 1功能丧失突变体atper 1 -1和atper 1 -2表现出抑制种子的初生休眠，并伴有种子中阿坝含量降低和GA含量增加。此外，atper 1突变体种子萌发过程中对非生物胁迫不敏感。与野生型种子相比，atper 1突变体种子中的几个阿坝催化剂基因（CYP 707 A1，CYP 707 A2和CYP 707 A3）和GA生物合成基因（GA 20 ox 1，GA 20 ox 3和KAO 3）的表达增加。通过缺失CYP 707 A基因，atper 1 -1种子的休眠被完全解除。此外，AtPER 1的功能缺失不能提高aba 2 -1或ga 1-t的种子萌发率，表明AtPER 1增强种子的初生休眠依赖于阿坝和GA。此外，atper 1突变体种子中的活性氧（ROS）水平显着高于野生型种子。综上所述，我们的研究结果表明，AtPER 1消除ROS抑制阿坝catalysts和GA的生物合成，从而改善了初级种子休眠，使种子对不利的环境条件不太敏感。
Seed is vital to the conservation of germplasm and plant biodiversity. Seed dormancy is an adaptive trait in numerous seed-plant species, enabling plants to survive under stressful conditions. Seed dormancy is mainly controlled by abscisic acid (ABA) and gibberellin (GA) and can be classified as primary and secondary seed dormancy. The primary seed dormancy is induced by maternal ABA. Here we found that AtPER1, a seed-specific peroxiredoxin, is involved in enhancing primary seed dormancy. Two loss-of-function atper1 mutants, atper1-1 and atper1-2, displayed suppressed primary seed dormancy accompanied with reduced ABA and increased GA contents in seeds. Furthermore, atper1 mutant seeds were insensitive to abiotic stresses during seed germination. The expression of several ABA catabolism genes (CYP707A1, CYP707A2, and CYP707A3) and GA biosynthesis genes (GA20ox1, GA20ox3, and KAO3) in atper1 mutant seeds was increased compared to wild-type seeds. The suppressed primary seed dormancy of atper1-1 was completely reduced by deletion of CYP707A genes. Furthermore, loss-of-function of AtPER1 cannot enhance the seed germination ratio of aba2-1 or ga1-t, suggesting that AtPER1-enhanced primary seed dormancy is dependent on ABA and GA. Additionally, the level of reactive oxygen species (ROS) in atper1 mutant seeds was significantly higher than that in wild-type seeds. Taken together, our results demonstrate that AtPER1 eliminates ROS to suppress ABA catabolism and GA biosynthesis, and thus improves the primary seed dormancy and make the seeds less sensitive to adverse environmental conditions.