Electrophysiological and morphological properties of neurons in the prepositus hypoglossi nucleus that express both ChAT and VGAT in a double-transgenic rat model.
Electrophysiological and morphological properties of neurons in the prepositus hypoglossi nucleus that express both ChAT and VGAT in a double-transgenic rat model.
复制标题
双转基因大鼠模型中表达 ChAT 和 VGAT 的舌下核神经元的电生理和形态学特性。
DOI：
10.1111/ejn.12878
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Yanagawa Y.
中科院分区：
文献类型：
--
作者：
Saito Y;Zhang Y;Yanagawa Y.
虽然已经提出含有乙酰胆碱（ACh）和γ-氨基丁酸（GABA）的神经元存在于舌下前置核（PHN）中，但由于难以识别，这些神经元尚未被表征。在本研究中，使用双转基因大鼠鉴定了同时表达胆碱乙酰转移酶和囊泡GABA转运蛋白（VGAT）的PHN神经元，其中胆碱能和抑制性神经元分别表达荧光蛋白tdTomato和Venus。为了表征同时表达tdTomato和Venus的神经元（D+神经元），通过脑干切片制备的全细胞记录研究了这些神经元的后超极化（AHP）特征和放电模式。关于三个层次分析法的配置文件和四个放电模式，D+神经元表现出，层次分析法与后去极化和放电模式，表现出延迟的第一个尖峰的产生是这些神经元的优先属性。在这三种形态类型中，多极型的D+神经元主要表现为放射状树突。免疫细胞化学分析显示，表达tdTomato的VGAT免疫阳性轴突终末主要位于下橄榄（IO）和PHN的背帽中。虽然PHN接受来自脚桥被盖核和背侧被盖核的胆碱能输入，D+神经元缺席这些脑区。总之，这些结果表明，共表达ACh和GABA的PHN神经元表现出特定的电生理和形态学特性，并支配IO和PHN的背帽。
Although it has been proposed that neurons that contain both acetylcholine (ACh) and γ‐aminobutyric acid (GABA) are present in the prepositus hypoglossi nucleus (PHN), these neurons have not been characterized because of the difficulty in identifying them. In the present study, PHN neurons that express both choline acetyltransferase and the vesicular GABA transporter (VGAT) were identified using double‐transgenic rats, in which the cholinergic and inhibitory neurons express the fluorescent proteins tdTomato and Venus, respectively. To characterize the neurons that express both tdTomato and Venus (D+ neurons), the afterhyperpolarization (AHP) profiles and firing patterns of these neurons were investigated via whole‐cell recordings of brainstem slice preparations. Regarding the three AHP profiles and four firing patterns that the D+ neurons exhibited, an AHP with an afterdepolarization and a firing pattern that exhibited a delay in the generation of the first spike were the preferential properties of these neurons. In the three morphological types classified, the multipolar type that exhibited radiating dendrites was predominant among the D+ neurons. Immunocytochemical analysis revealed that the VGAT‐immunopositive axonal boutons that expressed tdTomato were primarily located in the dorsal cap of inferior olive (IO) and the PHN. Although the PHN receives cholinergic inputs from the pedunculopontine tegmental nucleus and laterodorsal tegmental nucleus, D+ neurons were absent from these brain areas. Together, these results suggest that PHN neurons that co‐express ACh and GABA exhibit specific electrophysiological and morphological properties, and innervate the dorsal cap of the IO and the PHN.