Electrospun anti-inflammatory patch loaded with essential oils for wound healing
Electrospun anti-inflammatory patch loaded with essential oils for wound healing
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DOI：
10.1016/j.ijpharm.2020.119067
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发表时间：
2020-03-15
影响因子：
5.8
通讯作者：
Tasciotti, Ennio
中科院分区：
文献类型：
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作者：
Garcia-Salinas, Sara;Evangelopoulos, Michael;Tasciotti, Ennio
伤口愈合是一个复杂的过程，包括三个重叠的阶段：炎症，增殖和重塑。细菌感染可以增加炎症并延迟这一过程。微生物与先天免疫系统密切相关，如巨噬细胞和中性粒细胞，因为它们可以启动炎症级联反应。由于精油具有降低抗菌药物耐药性的能力，因此在抑制和预防细菌生长方面发挥着重要作用。找到一种结合抗菌和抗菌特性的策略的可能性对伤口愈合特别有吸引力。在这项工作中，我们展示了各种基于静电纺丝聚己内酯（PCL）纳米纤维的贴片，这些纳米纤维负载有来自精油的天然化合物，如百里酚（THY）和酪醇（TYR），以减少炎症。此外，我们比较了这些精油在加入PCL贴片时对活化巨噬细胞的影响。具体地，我们证明了当与PCL-TYR和含有TYR和THY的组合贴剂（即，PCL-TYR-THY）。此外，PCL-THY显示出对细胞附着的低亲和力，这可能阻碍伤口粘附和整合。总的来说，我们的研究结果表明，THY负载的贴片可以作为制造敷料的有前途的候选者，这些敷料具有杀菌和抗炎特性，同时避免了传统的载药装置的局限性。
Wound healing is a complex process that consists of three overlapping phases: inflammation, proliferation, and remodeling. A bacterial infection can increase inflammation and delay this process. Microorganisms are closely related to the innate immune system, such as macrophages and neutrophils, as they can start an inflammatory cascade. Essential oils play an important role in the inhibition and prevention of bacterial growth due to their ability to reduce antimicrobial resistance. The possibility to find a strategy that combines antimicrobial and antiinflammatory properties is particularly appealing for wound healing. In this work, we showcase a variety of patches based on electrospun polycaprolactone (PCL) nanofibers loaded with natural compounds derived from essential oils, such as thymol (THY) and tyrosol (TYR), to achieve reduced inflammation. In addition, we compared the effect these essential oils have on activated macrophages when incorporated into the PCL patch. Specifically, we demonstrate that PCL-THY resulted in more efficient down-regulation of pro-inflammatory genes related to the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa b) pathway when compared to PCL-TYR and the combination patch containing TYR and THY (i.e., PCL-TYR-THY). Furthermore, PCL-THY displayed low affinity for cell attachment, which may hinder wound adherence and integration. Overall, our results indicate that THY-loaded patches could serve as promising candidates for the fabrication of dressings that incorporate bactericidal and anti-inflammatory properties while simultaneously avoiding the limitations of traditional antibiotic-loaded devices.