Detection of 27 molecules in a single injection volume by hadamard transform capillary electrophoresis
Detection of 27 molecules in a single injection volume by hadamard transform capillary electrophoresis
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DOI：
10.2116/analsci.19.1659
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发表时间：
2003-12-01
影响因子：
1.6
通讯作者：
Imasaka, T
中科院分区：
文献类型：
--
作者：
Kaneta, T;Kosai, K;Imasaka, T
通过改进哈达玛变换毛细管电泳的实验装置，荧光素离子的浓度检测限达到了 100 fM。两个氩离子激光器，一个用于样品注射的门控激光器和一个用于激发分析物分子的探针激光器，用于使用光门控样品注射方法在激光诱导荧光检测中对分析物分子进行有效的光降解。此外，位于探测激光路径中的二向色镜用于排除氩离子激光的其他线。使用2046量级的Hadamard矩阵，在S/N = 3时确定荧光素离子的检测浓度限为100 fM，其中计算出单次注射体积中的平均分子数为27。还讨论了门控激光器和探测激光器的输出功率对灵敏度的影响。
A concentration detection limit of 100 fM was achieved for the fluorescein ion by improving the experimental setup used for Hadamard transform capillary electrophoresis. Two argon-ion lasers, a gating laser for sample injection and a probe laser for the excitation of analyte molecules, were employed for the efficient photodegradation of analyte molecules in laser-induced fluorescence detection using an optically gated sample-injection method. In addition, a dichroic mirror, located in the pathway of the probe laser was used to exclude the other lines of the argon-ion laser. Using a Hadamard matrix on the order of 2046, the concentration limit of detection for fluorescein ion was determined to be 100 fM at S/N = 3, in which the average number of molecules in a single injection volume was calculated to be 27. The influences of the output power in both the gating and probe lasers on the sensitivity are also discussed.