Flow cytometry analyses of adipose tissue macrophages.
Flow cytometry analyses of adipose tissue macrophages.
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DOI：
10.1016/b978-0-12-411619-1.00016-1
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发表时间：
2014
影响因子：
--
通讯作者：
Lumeng, Carey N.
中科院分区：
文献类型：
--
作者：
Cho, Kae Won;Morris, David L.;Lumeng, Carey N.
在脂肪组织中，多种白细胞相互作用有助于健康的代谢稳态以及胰岛素抵抗与肥胖的发病机制。脂肪组织巨噬细胞（ATMs）是脂肪中主要的白细胞群，有助于肥胖诱导的炎症。利用流式细胞术和流式辅助细胞分选技术对脂肪基质血管部分中的atm和其他白细胞进行表征。这些方法允许从啮齿类动物和人类的多个脂肪组织库中对这些独特的细胞群进行免疫分型、定量和纯化。适当的分离、定量和表征ATM表型对于理解它们在脂肪组织功能和肥胖诱导的代谢疾病中的作用至关重要。在这里，我们提出了流式细胞术在我们实验室使用的瘦和肥胖小鼠的atm表型分析方案。
Within adipose tissue, multiple leukocyte interactions contribute to metabolic homeostasis in health as well as to the pathogenesis of insulin resistance with obesity. Adipose tissue macrophages (ATMs) are the predominant leukocyte population in fat and contribute to obesity-induced inflammation. Characterization of ATMs and other leukocytes in the stromal vascular fraction from fat has benefited from the use of flow cytometry and flow-assisted cell sorting techniques. These methods permit the immunophenotyping, quantification, and purification of these unique cell populations from multiple adipose tissue depots in rodents and humans. Proper isolation, quantification, and characterization of ATM phenotypes are critical for understanding their role in adipose tissue function and obesity-induced metabolic diseases. Here, we present the flow cytometry protocols for phenotyping ATMs in lean and obese mice employed by our laboratory.