STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis.
STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis.
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DOI：
10.1093/nar/gks1309
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发表时间：
2013-02-01
影响因子：
14.9
通讯作者：
Lee BH
中科院分区：
文献类型：
--
作者：
Ben Chaabane S;Liu R;Chinnusamy V;Kwon Y;Park JH;Kim SY;Zhu JK;Yang SW;Lee BH
microRNAs（miRNAs）是一类小分子调控RNA，在许多真核生物的发育和代谢过程中发挥重要的调控作用。在拟南芥中，DICER-LIKE 1（DCL 1）、HYPONASTIC LEAVES 1、SERRATE、HUA ENHANCER 1和HASTY参与初级miRNA（pri-miRNA）的加工以产生前体miRNA（pre-miRNA）并最终产生miRNA。除了这些成分，mRNA帽结合蛋白，CBP 80/阿坝超敏感1和CBP 20，也参与miRNA的生物合成。在这里，我们表明，STABILIZED 1（STA 1），拟南芥前mRNA加工因子6同源物，也参与了miRNA的生物合成。与其他miRNA生物发生缺陷突变体相似，sta 1 -1积累了显著较低水平的成熟miRNA，同时比野生型积累了更高水平的pri-miRNA。成熟miRNAs的急剧减少与其靶基因转录本的积累和发育缺陷有关。此外，sta 1 -1损害了含有pri-miRNAs的内含子的剪接，并降低了DCL 1的转录水平。这些结果表明STA 1通过在pri-miRNA剪接中发挥作用直接参与miRNA生物合成，并通过调节DCL 1转录水平间接参与miRNA生物合成。
MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processing of primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesis-defective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.
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