Toxicity of cadmium in tobacco smoke:: Protection by antioxidants and chelating resins
Toxicity of cadmium in tobacco smoke:: Protection by antioxidants and chelating resins
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DOI：
10.1080/10715760210159
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发表时间：
2002-01-01
影响因子：
3.3
通讯作者：
Polla, BS
中科院分区：
文献类型：
--
作者：
Bachelet, M;Pinot, F;Polla, BS
镉是一种存在于烟草烟雾中的环境毒素，我们在体外对人类单核细胞进行了研究，并与烟草烟雾的影响进行了比较。在暴露于镉或烟草烟雾的人单核细胞中，72 kDa热休克/应激蛋白Hsp70的过表达和细胞死亡具有相似的时间过程和相似的程度。镉和烟草烟雾介导的毒性与细胞中谷胱甘肽和ATP含量的降低有关，而谷胱甘肽前体n -乙酰- l-半胱氨酸可以阻止镉和烟草烟雾介导的毒性。此外，镉螯合剂树脂Chelex-100预处理可防止烟草烟雾介导的毒性，支持镉在烟草烟雾介导毒性中起主要作用的结论。
The effects of cadmium, an environmental toxin present in tobacco smoke, were studied in vitro in human monocytes and compared to those of tobacco smoke. Overexpression of the 72 kDa heat shock/stress protein Hsp70 and cell death occurred with a similar time-course and to a similar extent in human monocytes exposed to either cadmium or tobacco smoke. Cadmium and tobacco smoke-mediated toxicity were associated with a decrease in the cellular content of glutathione and ATP and the glutathione precursor N-acetyl-L-cysteine prevented both cadmium and tobacco smoke-mediated toxicity. Furthermore, tobacco smoke-mediated toxicity was prevented by pretreatment with the cadmium chelator resin Chelex-100, supporting the conclusion that cadmium plays a major role in tobacco smoke-mediated toxicity.